基质金属蛋白酶MMP-2、MMP-9在后发性白内障形成中的变化

Different expression of MMP-2 and MMP-9 in formation of posterior capsule opacification

目的：探讨兔眼晶状体皮质吸出术后不同时期MMP-2、MMP-9的变化,推断其在后发性白内障形成过程中的作用.方法：选用中国医科大学实验动物部提供的白色家兔42只,随机分成实验组（35只）及对照组（7只）.实验组肌肉注射氯胺酮15mg/kg进行麻醉,由专人行双眼透明晶状体皮质吸出术.术后1%阿托品眼膏散瞳双眼,分别于术后1周、1、2、3、5个月各处死7只兔;对照组不做处理,直接处死.石蜡标本进行免疫组织化学定位检测MMP-2、MMP-9,冷冻标本用Zymography方法进行MMP-2、MMP-9活性测定.结果：①兔眼后发性白内障形成情况：术后不同时期裂隙灯下观察可见术后前3d前房内炎症反应明显,虹膜充血;术后1周可见前囊截缘出现白色环状混浊,但后囊尚透明;术后2周后囊呈薄纱状;1个月出现线条状混浊,2～3个月成条索状混浊,术后5个月后囊明显混浊.②免疫组化结果：对照组晶体上皮细胞胞浆及细胞间质中没有明显的MMP-2、MMP-9表达,实验组MMP-2于术后1～3个月表达增强.③Zymography凝胶检查：对照组可检测到极低的MMP-2、MMP-9活性（呈淡条带）;实验组MMP-2活性明显强于对照组,MMP-9无明显差异.④活性分析：对照组MMP-2活性为（0.75±0.10）,实验组分别为（3.68±0.12）、（5.18±0.11）、（5.62±0.11）、（6.58±0.11）和（1.19±0.10）;实验组MMP-9活性与对照组相比无显著性差异.结论：正常兔晶体囊膜中有MMP-2、MMP-9活性;术后2～3个月囊膜中MMP-2活性增强;术后2～3个月,囊膜中MMP-9无变化;MMP-2与MMP-9比较,前者与后发性白内障的发病关系更密切.

Objective To discuss the changes of MMP - 2 and MMP - 9 level in different stages after cortex exsuccation of lens in rabbits and their effect in the formation of posterior capsule opacification. Method Forty - two healthy white rabSits from the experimental animal department of CMU were divided into the test group （ n = 35 ） and the comparison group （ n = 7 ） randomly. Ketamine was intramuscular injected for anaesthesia （15mg/kg） and cortex exsuccation of lens was performed on two eyes in the test group, mydriasis with 1% atropine after operation. 7 rabbits were executed 1 week, 1 month,2 months ,3 months and 5 months after the operation respectively. Rabbits in the comparison group were executed with no operation. Zymography test and immunohistochemical technique wen used separately to determine the activity of MMP - 2 and MMP - 9 of the paraffine sample and the refrigeration sample. Results （1）The formation of posterior capsule opacification in rabbit eyes： observation in different stages after the operation showed the inflammatory response remarkable at the first three days with iris congestion; edge of anterior capsule circularity opacification at the 1 st week, but no remarkable change in the posterior capsule; just gauze -like at the 2nd week; strip -like opacification at the end of the 1 st month, funicular opacification after ：2 - 3 months, while visible opacitication at the 5th month after the operation. （2）Results of immunohistochemical technique： there was no obvious expression of MMP - 2 and MMP - 9 in lens epithelia and stroma in the comparison group, while higher expression of MMP - 2 in 1 - 3 months after the operation in the test group. （3）Zymography test： low expression of MMP -2 and MMP -9 in the comparison group;the activity of MMP -2 in the test groups was stronger than that of the comparison group; but there was no remarkable difference of MMP -9. （4） Analysis of activity： the activity of MMP -2 in the comparison group was （0.75 ±0.10）, and （3.68 ±0.12） ,（5.18 ±0.11） ,（5.62 ± 0. 11 ）, （ 6.58 ± 0. 11 ）, （ 1.19 ± 0.11 ） in the test groups respectively. But there was no remarkable difference of MMP - 9 between the two groups. Conclusion MMP - 2 and MMP - 9 expression is found in normal lens capsule and MMP - 2 expresses highest in 2 - 3 months after cortex exsuccafion of lens. No change in the expression of MMP - 9 in lens capsule 2 - 3 months after operation and MMP - 2 plays a more important part in the formation of posterior capsule opacificafion than MMP - 9.