猕猴桃钙调蛋白cDNA的克隆及测序

Cloning and Sequencing of a Calmodulin cDNA from Kiwifruit

以“秦美”猕猴桃果实呼吸跃变初期ｃＤＮＡ第一链为模板，参考大麦钙调蛋白基因序列合成５′端和３′端引物，利用多聚酶链式反应（ＰＣＲ）合成了完整的猕猴桃钙调蛋白ｃＤＮＡ，克隆并测定了其全序列。结果表明，猕猴桃钙调蛋白基因读码框架内序列由４４４个核苷酸组成，共编码１４８个氨基酸，且与植物领域里发表的大麦及苜蓿的钙调蛋白基因有很高的同源性。在核苷酸序列上与大麦有８６．１６％的同源性，与苜蓿有８５．６９％的同源性，其编码的氨基酸与大麦和苜蓿的不同分别只是一个氨基酸的差异，同源率高达９９．３％．

The cDNA of calmodulin from kiwifruit ( Actinidia deliciosa C.F. Liang et A.R. Ferguson cv.Qinmei) was amplified and cloned using PCR with the first strand of cDNA of kiwifruit fruit as the template. Two primers of the 5' and 3' ends were composed with the reference to the sequence of acalmodulin gene from barley. The cDNA we cloned contained 444 nucleotides coding for 148 amino acid residues. The DNA sequence and deduced amino acid sequence reported here was 86.16% and 85.69% homologous compared with those in barkey and alfalfa respectively with the homologous rate of 99.3%.