猪大肠埃希菌高密度发酵条件的试验研究

Study on Conditions of High Density Fermentation in Piglet Escherichia coli

利用5 L发酵罐分批培养O2、O8、O135、O138、O139、O141血清型组成的猪大肠埃希菌菌液,通过活菌计数的方法,对大肠埃希菌在发酵过程中温度、pH、通气量、葡萄糖加量等基本条件进行试验研究.结果表明,在用发酵罐进行大肠埃希菌多个血清型的高密度发酵时,利用常规的干粉培养基,将培养温度控制在37℃±0.5℃,pH控制在7.0～7.2,逐渐增大通气量,根据pH的变化补加40%的葡萄耱,可以使细菌的培养菌数（cfu）达到150亿/mL以上.

Several batches Piglet E. coli （O2, O8, O135, O138, O139, O141 serotype） were cultivated in 5 liters Fermentor. The basic culture conditions such as temperature, pH, ventilation quantity and glucose content were analyzed by means of plating count and optimized. The results showed that Piglet E. coli grow best in routine power medium when the culture temperature was at 37 ＂C !0. 5 ℃ ,pH controlled in the range 7.0-7.2, ventilation volume progressively, add 40% concentration glucose solution according to the change of pH, the colony form unit （efu） of E. coli can reach 150 hundred million per ml.