用RAPD分析鉴定葡萄属远缘杂种

Identification of Hybrids Derived from Vitis rotundifolia× Vitis vinifera by RAPD Analysis

用随机扩增多态型ＤＮＡ（ＲＡＰＤ）技术，在幼苗期鉴定了圆叶葡萄和真葡萄亚属杂交的杂种。结果表明，以两个杂交组合后代及其亲本ＤＮＡ作模板，用随机引物ＵＢＣ－２５０，ＵＢＣ－２６６和ＵＢＣ－２７２可以分别扩增出１４００，１４４０，３５０和５００ｂｐ的多态性片段。

The identification of hybrids derived from Vitis rotundifolia×Vitis vinifera cultivar was carried out using RAPD techniques during seedling stage. The results showed the DNA polymorphisms of 1400,1440,350 and 500 bp were amplified by random primers of UBC 250,UBC 266,and UBC 272 and targeted to the molecular markers from seedless parents when the hybrids DNA were used as the templates from two cross combinations.Therefore,the hybrids,of both true and false would be determined by these molecular genetic markers before fruit production.