经济林木试管茎尖嫁接的研究现状及展望

Present Research Situation and Prospects of Shoot Apex Grafting in Economic Trees

根据经济林木试管茎尖嫁接近２０年来的研究成果，提出了今后的重点研究领域。早期的病毒脱除和快速繁殖已由研究阶段走向应用，在经济林木嫁接不亲和性的早期诊断和诱导发育阶段返幼方面个别树种有所突破，不少技术与理论问题有待研究解决，而对于发育阶段返幼的发生机理则迄今人们知之甚少。今后的工作拟集中于：①多种经济林木的脱毒与快繁；②通过生理、生化和组织细胞学观察，认识不同发育阶段的内在变化。

Based on the experiment achievement of shoot apex grafting in economic trees in the last 20 years, the key field for the future research has been pointed out. The virus freeing and high speed propagating in the early stage has changed from research period to application period. The early diagnosis of incompatibility of graft and rejuvenation have been broken through in some species . But the techniques and theories, especially in rejuvenation, still need to be studied further, which include ①virus free and high speed propagation in different kinds of economic trees;②discovery of the causes of the rejuvenation through biophysics, biochemistry and cytological observations, and through the inner change understanding during different growth periods.