生、炒酸枣仁HPLC指纹图谱比较

Comparative studies on the HPLC fingerprints of the Semen Ziziphi Spinosae(SZS) and the parched Semen Ziziphi Spinosae(pSZS)

目的:采用高效液相色谱法建立生、炒酸枣仁的指纹图谱,进行成分对比。方法:Hanbon C1(84.6mm×250mm,5μm)色谱柱,乙腈-0.05%三氟乙酸(TFA)梯度洗脱,流速1.0mL/min,检测器为蒸发光散射检测器(ELSD)与二极管阵列检测器(DAD)串联使用,建立酸枣仁指纹图谱的分析方法。结果:生、炒酸枣仁醇提物的中等极性部分的HPLC指纹图谱基本一致。结论:生、炒酸枣仁醇提物的中等极性部分的化学成分未见显著差异,推断其炮制前后成分的差别可能存在于极性强的水溶性部分或极性弱的脂溶性部分。

Objective：To study the difference of HPLC fingerprints of the chemical component between the SZS and pSZS. Methods：The experimental conditions of the HPLC method were as follows：Hanbon C18 column （4.6mm×250mm,5μm）, mobile phase consisting of acetonitrile-0.05% TFA in gradient mode,flow rate at 1.0 mL/min,thc detectors of evaporative light scattering detector （ELSD） and photodiode array detector （DAD） connected in series.Results：The HPLC fingerprints of SZS and pSZS are almost the same.Conclsion：There have no significant deviation between semipolarity components of the SZS and pSZS, and the different components may be relate to the tiposolubility part and water-solubility part.