摘要
通过用聚丙烯封口膜转接玻璃化苗,以及将玻璃化苗放在自然光下进行处理,和在培养基中加入不同浓度的糖、琼脂、细胞分裂素(6-BA)进行正交试验3种处理,得出百里香玻璃化苗的最佳逆转方式是,将其接入培养基为MS+6-BA 1.5?/L+IBA 0.1?/L+10 g/L琼脂+50 g/L蔗糖的培养基中进行逆转培养,逆转率为66.7%。
Effect of polypropylene sealing material,illumination,media containing different phytohormones and natural substances on the the vitrification of plantlets of thymes was studied by orthogonal design.The experimental results showed that sugar is the main factor in reversing the vitrification and the best media for controlling vitrification of thyme plantlet with the reversion rate is up to 66.7% is MS ＋ 6-BA 1.5㎎/L ＋ IBA 0.1㎎/L＋10 g/L agar ＋ 50 g/L sugar.
出处
《内蒙古农业大学学报（自然科学版）》
CAS
2007年第2期205-208,共4页
Journal of Inner Mongolia Agricultural University(Natural Science Edition)
基金
北京林业大学自选课题资助项目(05jjo59)
关键词
百里香
玻璃化
逆转
Thymus serpyllum L.var.mongolicus
vitrification reversion
