基质金属蛋白酶-9与自发性高血压大鼠心肌纤维化的相关性研究

Correlative Research of Matrix Metalloproteinases-9 and Myocardial Fibrosis of Spontaneously Hypertensive Rats

目的:探讨基质金属蛋白酶-9(MMP-9)与自发性高血压大鼠(SHR)心肌纤维化的相关性。方法:将16只14周龄雄性SHR随机平分为ACEI组和对照组,另以8只同龄雄性SD大鼠作为正常对照组。ACEI组以卡托普利100mg/(kg.d)灌胃,SHR对照组不灌药,于灌药12周后麻醉下取出大鼠心脏。免疫组化分析MMP-9、Collagen和的表达;RT-PCR检测MMP-9mRNA表达;MASSON染色测量胶原容积分数(CVF);碱水解法测定羟脯氨酸(Hypro)含量。结果:(1)SHR对照组LVI、CVF、Hypro、MMP-9、Collagen和的表达明显高于正常对照组(P<0.01);相对于SHR对照组,ACEI组各参数则显著降低(P<0.05);(2)MMP-9与上述指标呈高度正相关(P<0.01)。结论:MMP-9与SHR心肌纤维化密切相关,ACEI能抑制MMP-9的表达。

Objective：To investigate the correlation of matrix metalloproteinases-9（MMP-9 ）and myocardial fibrosis of Spontaneously Hypertensive Rats （SHRs）. Methods：16 fourteen-week-old male SHRs were randomly assigned to ACEI and control group equivalently. 8 same age male SD rats were as normal control group. A daily dose of 100 mg/ （kg · d） captopril were given to ACEI group for 12 weeks by gavage. MMP-9.Collagen Ⅰ and Ⅲ were evaluated by immunohistochemistry and RT-PCR;CVF was measured by MASSON staining;The concentration of Hypro was detected by Alkaline hydrolysis method. Results：The LVI.CVF.Hypro and the expression of MMP-9.Collagen Ⅰ and Ⅲof SHR control group were significantly higher than those of normal control group （P〈0. 01）. Compared with SHR control group, these indices of ACEI group decreased obviously（P〈0.05）. Conclusions：MMP-9 is closely connected with myocardial fibrosis of SHR and suppressed by ACEI.