基于壳聚糖载体的蛋白质药物纳米颗粒制备研究

Preparation of Chitosan Nanoparticles As Protein Drug Carriers

采用基于壳聚糖(CS)与聚阴离子(多聚磷酸纳)间静电作用的离子凝胶化方法,以牛血清白蛋白(BSA)为模型,在室温下制备了包载蛋白质的亲水性壳聚糖纳米颗粒.对BSA-壳聚糖纳米颗粒的形成条件进行了考察,结果表明:在pH值为5.0,CS与TPP的质量比为4,壳聚糖分子量为40 kDa的最优化的条件下可制备粒径小于100 nm的BSA-壳聚糖纳米颗粒,对BSA的包封率达到50%以上.并将该体系初步应用于蛋白类药物丙种球蛋白-壳聚糖纳米颗粒的制备研究,这种壳聚糖纳米颗粒对丙种球蛋白具有良好的缓释作用.

A new type of hydrophilic chitosan nanoparticles encapsulated with BSA （BSA-CS NPs） was prepared through ionic cross-linking technique based on CS and sodium tripolyphosphate （TPP） at room temperature. The influence of formulation conditions on the size of the BSA-CS NPs was investigated. Results showed that BSA-CS NPs with diameter of below 100 nm were prepared when the pH value was 5.0, the mass ratio of CS to TPP was 4, the molecular weights of CS was 40 kDa, and the BSA encapsulation efficiency was more than 50%. This system was also applied in the preparation of chitosan nanoparticles encapsulated with Gamma Seroglobulin （IG-CS NPs）.