持续温热腹腔灌注对大鼠外周血NK细胞活性和腹腔器官的影响

Effects of Continuous Intraperitoneal Hyperthermic Perfusion on Peripheral NK Cytolytic Activity and Peritoneal Viscera in Rats

通过在大鼠建立温热灌注模型，研究了（42±1）℃、1h温热生理盐水腹腔持续灌注对外周血NK细胞活性和腹腔内器官的影响。结果发现温热灌注后外周血NK细胞活性短暂上升，2周时恢复正常。术后2周内可见脾小体普遍增大，动脉周围淋巴鞘增宽。腹腔内无明显粘连。肝、肾无肉眼可见病变。术后1d时血浆谷丙转氨酶显著升高，2周内恢复。白蛋白术后下降较快。术后1d时镜下见肝浅层细胞胞浆疏松化，5d时正常。2（2／10）例术后1d时肠粘膜糜烂，2周可恢复。结果表明（42±1）℃、1h腹腔温热灌注可增强大鼠NK细胞杀伤活性，对术后早期抗肿瘤免疫可产生有利影响。同时对大鼠腹内器官损伤作用轻微。易受热损伤的器官为肠粘膜及肝脏。

An animal model of intraperitoneal hyperthermic per fusion was established in Rats. Investigation was carried out to recognize the effects of 42±1℃, 1 hr. peritoneal perfusion on peripheral natural killer cytolytic activity and injuries to peritoneal viscera. It was found that activity of NK cells rose on day 5after hyperthermia and went down to normal in the 2nd week. Lymph nodules in the spleen showed difusive hypertrophy and the periarterial lymphocyte sleeve widened in 2 weeks- Peritoneal adhesion was not observed. Serum GPT level jumped up on day 1 and stepped down nearly to normal in 2 weeks. Serum albumin level was significantly decreased. Accordingly, cells close to liver capsule swelled balloon-likely on day 1 after hyperthermia and recovered by day 5. Two Rats showed intestinal mucosa erosive lesions on day 1 after hyperthermia, but no lesions were observed in the 2nd week. These results showed that 42±1℃, 1 hr. in traperitoneal perfusion could enhance the cytolytic activity of NK cells. Beneficial effect on early postoperative antitumor immunity could be obtained from this event. It was also proved that injuries to abdominal organs were not severe during the 42±1℃, 1 hr. intraperitoneal hyperthermia. Organs susceptible to heat are intestinal mucosa and liver.