摘要
目的 观察氟对小鼠神经细胞内游离钙(Ca^2+)的影响,为探讨氟中毒的发生机制提供实验依据。方法 ①采用细胞培养方法,体外培养小鼠神经细胞。根据加氟剂量(NaF,0、5.0、10.0、15.0、20.0、40.0mg/L)不同分为6组,在培养第15天,收集各组细胞,激光扫描共聚焦显微镜记录细胞内Ca^2+荧光图像;②出生后7d小鼠,按腹腔注射不同剂量的氟溶液(0、0.7、2.8、11.2mg/kg)分为4组,于注射后8h处死小鼠,收集脑细胞,制备细胞悬液,记录细胞内Ca^2+荧光图像。结果 体外培养加氟(≥5.0mg/L)和体内腹腔注射氟溶液(≥0.7mg/kg)均可导致神经细胞内Ca^2+增加,组间比较差异有统计学意义(F=191.20、93.83,P〈0.01);各加氟组细胞内Ca^2+水平与对照组比较,差异均有统计学意义(P〈0.01)。结论 氟诱导神经细胞内Ca^2+增加,且随着加氟剂量的增加,细胞内Ca^2+水平也增加,二者呈剂量效应关系。
Objective To observe neurotoxicity caused by fluoride in order to provide an experimental basis for studying the mechanism of fluorosis. Methods ①Neuron were cultured at different doses of fluoride (0,5.0,10.0,15.0,20.0,40.0 mg/L). Neurons were recollected on the 15th day of cell culture to observe morphology. Ca^2+ in neuron was inspected with confocal laser scanning microscopy; ② Wistar newborn rats were injected intraperitoneally with different doses of fluoride (0,0.7,2.8,11.2 mg/kg); 8 hours later, rats were killed for collecting brain cells and making cell suspension. Ca^2+ in neurons were then inspected with confocal laser scanning microscopy. Results In vitro culture in Flouride medium(≥5.0 mg/L)or introperitoneal injection of fluoride (≥10.7 mg/kg) could induce the intracellular Ca^2+ to increase in mice with fluorosis, the difference being significant between groupos (F= 191.20,93.83, P 〈 0.01). Conclusion Flouride induces intracellular Ca^2+ to increase in a dose-dependent manner.
出处
《中国地方病学杂志》
CAS
CSCD
北大核心
2007年第5期505-507,共3页
Chinese Jouranl of Endemiology
基金
兰州市科技局科技发展计划项目(041206)
关键词
氟
脑细胞
细胞培养
游离钙
Flouride
Brain cell
Cell, cultured
Intracellular Ca^2+