摘要
目的 观察尼克酰胺腺嘌呤二核苷酸（NADH）对阿霉素（ADR）损伤的大鼠心肌保护作用。方法 将60只雄性健康Wistar大鼠随机分为ADR组、NADH组、ADR＋NADH组及对照组。在实验第8周处死大鼠，光镜下观察心肌形态结构及病理改变：用生化方法测定大鼠心肌组织、心肌细胞线粒体中谷胱甘肽过氧化物酶（GSH—Px）、超氧化物歧化酶（SOD）活性及丙二醛（MDA）水平。结果 光镜下ADR＋NADH组大鼠心肌组织损伤程度较ADR组明显减轻；从心肌组织GSH—Px、SOD活性可以看出，与ADR组[（3．97±1．11，15．63±3．79）kU／gPr]比较，ADR＋NADH组[（5．88±0．97，49．27±4．22）kU／gPr]明显增高，二者比较差异有统计学意义（q=7．22、32．18，P〈0．01）；心肌组织MDA水平，ADR组[（4．55±1．51）μmol／g Pr]明显高于ADR＋NADH组[（3．64±0．75）μmoL／gPr]，二者比较差异有统计学意义（q=8．48，P〈0．01）。结论 NADH对阿霉素性心肌损伤有一定的保护作用．这种保护作用可能主要与NADH提高受损心肌细胞抗氧化能力有关。
Objective To study the protective effects of nicotinamide adenine dinucleotide-reduced（NADH） on myocardium injury caused by adriamycin （ADR）. Methods Sixty male Wistar rats were randomly divided into four groupscontrol, NADH, ADR ＋ NADH and ADR groups. In the 8th week the animals were killed by exsanguinations. Myocardium was observed under light microscope. Malondialdehyde （MDA） level and activity of glutathione peroxidase （GSH-Px） and superoxide dismutase（SOD） of myocardium were histopathologically measured. Results The extent of myocardial injury of ADR ＋ NADH group were lower than that of ADR group. Activities of myocardial GSH-Px and SOD of ADR ＋ NADH group were significantly higher than those of ADR group （3.97 ± 1.11 vs 5.88 ± 0.97, P 〈 0.01 ; 49.27 ±4.22 vs 15.63 ± 3.79, P 〈 0.01 ）. Myocardial MDA level of ADR ＋ NADH group was obvious lower than that of ADR group （3.64± 0.75 vs 4.55 ± 1.51, P 〈 0.01）. Conclusion NADH could protect against ADRinduced myocardial injury possibly via increasing antixidation of cadiocytes.
出处
《中国地方病学杂志》
CAS
CSCD
北大核心
2007年第5期527-529,共3页
Chinese Jouranl of Endemiology
基金
黑龙江省科技攻关课题（GB05C401-11）
