CoulTER Jen's检测网织红细胞参数临床意义分析

Clinical Significance of Detecting Reticulocyte Parameters by CoulTER Jen's Blood Analyzer

目的通过对CoulTER Jen's型血液分析仪测定的强光反射网织红细胞(High Laser Reticuloyte,HLR)参数做相关调查,探讨网织红细胞参数在各类贫血患者中的应用及意义。方法调查80份正常人血液标本,统计其参考范围,调查110份贫血标本,观察HLR的平均值,观察不同类型贫血HLR的差异。同时做网织红细胞成熟度(IRF)的统计分析,观察与HLR的相关情况。结果正常人的HLR平均值为0.42%,贫血标本的HLR的均值为1.88%。再生障碍性贫血、急性白血病、溶血性贫血、缺铁性贫血、巨幼红细胞贫血、慢性肾炎和恶性肿瘤的HLR平均值依次分别为0.11%、0.32%、3.2%、0.88%、1.06%、0.86%、1.26%。结论观察网织红细胞参数对于贫血患者的诊断、判断病情的发展和预后、指导临床用药及药物疗效有一定参考价值,比Hb降低敏感,其中IRF和HLR比RET%更敏感,且全自动仪器操作更准确。

Objective To investigate the clinical significance of the lens reflex reticulocyte （High Laser Reticulocyte, HLR） parameters detected by coulter Jen＇s blood analyzer in patients with various types of anemia, and explore the application range. Methods The reference scope was made statistically by 80 normal blood samples. The mean value of HLR was observed in 110 specimens from anemia patients, and the difference among different types of anemia was compared. The reticulocyte maturity （IRF） and its relevance to HLR were also analyzed statistically. Results The mean value of HLR was 0.42% from normal control and 1.88% from anemia patients. The mean value of HLR from aplastic anemia,acute leukemia, hemolytic anemia, iron-deficiency anemia, megaloblastic anemia, chronic nephritis and cancer were 0. 11%, 0.32%, 3.2%, 0.88%, 1.06%, 0.86%, 1,26% respectively. Conclusions The detection of reticulocyte parameters is helpful to the diagnosis of anemia patients,judging for the severity and prognosis and guiding clinical administer. The sensitivity and accuracy of IRF and HLR was higher than that of RET.