好氧生物降解改进的MITI(Ⅰ)试验方法研究

Evaluation of Aerobic Biodegradation Modified MITI(Ⅰ) Test

好氧生物降解改进的MITI(日本通商产业省)(Ⅰ)试验是用来评估化学品在特定温度条件下是否具有快速生物降解性的有效方法。以环境内分泌干扰剂壬基酚为受试物,应用改进的MITI(Ⅰ)试验对其生物降解性进行研究,亦对试验所需污泥进行驯化培养,并对污泥特性进行检测。试验结果表明处理28d后,壬基酚生物降解率为51.4%,不具有快速生物降解性,参比物苯甲酸钠生物降解率为89.1%。在污泥培养过程中,污泥重量、体积变化规律为随培养期的增加污泥重量、体积逐渐增大,后逐渐趋于稳定。污泥活性的变化规律为随培养期增加,污泥活性先增强,后逐渐趋于稳定。

Modified MITI（ I ） test is one of an important aerobic biodegradation test for the＇determination of ready biodegradability of chemical compounds in the special temperature. Biodegradation of one of the environmental endocrine -disrupting chemicals 4-NPC under the modified MITI（ I ） test was studied. In this research, we also cultivated sludge and determined the characteristic. The results of the research showed that the percentage biodegradation of 4-NPC calculated from the oxygen consumption was 51.4% after 28 days, so that it had not ready biodegradability. The percentage biodegradation of sodium benzoate was 89.1%. During the period of sludge cultivation, the weight, volume and activity of the sludge were increased gradually at first and tend to changeless.