非洲菊离体快速繁殖技术

Tissue Culture Protocol for Quick Propagation of Gerbera jamesonii Bolus

以非洲菊品种107-2幼嫩花蕾为外植体进行离体快速繁殖技术研究。结果表明:外殖体诱导愈伤组织并分化不定芽的适宜培养基为1/2 MS+10.00 mg·L-1 BA+0.50 mg·L-1 IAA,培养10 d以后,愈伤组织诱导率达100%,培养60 d以后,芽的分化率达93.5%;芽增殖的适宜培养基为MS+0.2 mg·L-1 BA+5.0 mg·L-1 KT+0.3 mg·L-1 IAA,培养30 d以后,芽的增值系数为2.5,苗体生长健壮、翠绿,小苗高度可达2.3 cm;壮苗生根的适宜培养基为1/2 MS+0.15 mg·L-1IAA,培养22 d以后,生根率可达95%,每株苗平均根数为3.6条,平均根长为1.2 cm,小苗植株翠绿挺拔,根系粗壮,移栽后都能成活。

A protocol for quick propagation was set up using young flower buds of Gerbera jamesonii Bolus cv. 107-2 as the explants for tissue culture. Results showed that the combination of 1/2 MS＋10.00 mg.L^-1 BA ＋0.5 mg.L^-1 IAA was a suitable medium for callus induction and adventitious bud differentiation, callus induction rate being as high as 100% after 10 d induction and bud differentiation rate as 93.5% after 60 d induction. MS supplemented with 0.2 mg.L^-1 BA ＋5.0 mg.L^-1 KT ＋ 0.3 mg.L^-1 IAA appeared to be desirable for subculture and multiplication, the bud multiplication coefficient being 2.5, planflets being strong in green and the planflet height reaching 2.3 cm after 30 d induction. The optimum rooting medium was 1/2 MS ＋ 0.15 mg.L^-1 IAA, the rooting rate being 95% with an average roots per plant being 3.6 and root length being 1.2 cmafter 22 d culture. The plantlets all survived well after transplantation.