海洋来源真菌烟曲霉中胶霉毒素类成分研究

Studies on the Chemical Constituents of Gliotoxins Produced by Marine-derived Aspergillus Fumigatus

为了从海洋来源的微生物中寻找新的抗肿瘤活性天然产物,对一株具有较强细胞毒活性的海洋来源真菌烟曲霉进行系统分离研究,以抗肿瘤活性为指标,采用硅胶柱层析、葡聚糖凝胶LH-20和HPLC等方法从中分离出4个单体化合物,经波谱数据分析及与文献对照分别鉴定为gliotoxin(1),bisdethiobis(methylthio)gliotoxin(2),d idehydrobisdethio-bis(methylthio)gliotoxin(3)和bis-N-norgliovictin(4),其中化合物(1)在0.15 mg/L浓度下对温敏型小鼠乳腺癌细胞(tsFT 210)显示了很强的细胞毒活性.

In order to find bioactive compounds from marine microorganisms, the secondary metabolites of the fermentation of aspergillus fumigatus were studied. Four compounds were isolated from the active extract by chromatography on silica gel, sephadex LH-20 column and HPLC, respectively. Their structure were identified on the basis of comparison to literature and spectroscopic analysis as gliotoxin（1）, bisdethiobis（methyhhio） gliotoxin（2）, didehydrobisdethio-bis（methylthio） gliotoxin（3） and bis-N-nor- gliovictin（4）. Compound （1） exhibited very strong cytotoxicity against tsFT 210 cell line at 0.15 mg/L.