湖羊6号染色体微卫星标记多样性与产羔数的关系

Relationship between genetic diversity of chromosome 6 determined by microsatellite markers and litter size in Hu sheep

选择位于绵羊6号染色体上FecB基因紧密连锁的6个微卫星标记,即LSCV043、BMS2508、GC101、300U、Bulge5和471U,分析其在湖羊中的遗传多样性以及和产羔数的关系。结果表明,6个微卫星位点均属多态性位点,共检测到34个等位基因、53种基因型。LSCV043、BMS2508和300U属高度多态位点,其多态信息含量分别为0.6674、0.6035和0.5615。对不同基因型群体的产羔率进行统计分析,LSCV043基因型为107 bp/123 bp所对应的总体产羔数明显高于110 bp/123 bp基因型群体所对应的产羔数(P<0.05);BMS2508基因型为154 bp/154 bp、154 bp/170 bp和154 bp/200 bp所对应的群体第一胎产羔数明显高于170 bp/170 bp所对应的产羔数(P<0.05),基因型170 bp/170 bp的群体总体产羔数均低于该位点的其他基因型(P<0.05);其他位点各基因型之间产羔数均无显著差异。

Genetic diversity and relationship with litter size were analyzed in Hu sheep using 6 microsatellite markers LSCV043, BMS2508, GC101, 300U, Bulge5, and 471U, which were closely linked to FecB gene. Thirty-four alleles and 53 genotypes were detected at 6 microsatellite loci, which were all polymorphic. Three markers LSCV043, BMS2508 and 300 U were highly polymorphic loci. The polymorphism information content （PIC） were 0.6674, 0.6035, and 0.5615, respectively. The total mean of litter size of genotype LSCV043 107 bp/123 bp was significantly higher than that of genotype 110 bp/123 bp. The litter size of the fast lambing for genotypes BMS2508 154 bp/154 bp, 154 bp/170 bp and 154 bp/200 bp were significantly higher than that of genotype 170 bp/170 bp （P〈0.05）. Furthermore, total litter size for BMS2508 170 bp/170 bp was the lowest in all the genotypes within this locus （P〈0.05）. No significant difference was detected in other genotypes.