选择性逆向冠状静脉搭桥术后一氧化氮和内皮素的变化

Changes of Nitric Oxide and Endothelin After Selective Retrograde Coronary Venous Bypass

目的探讨选择性逆向冠状静脉搭桥术对犬心肌缺血模型一氧化氮和内皮素的影响及其意义。方法16只健康成年杂种犬,随机分成缺血组(n=8)和选择性逆向冠状静脉搭桥组(n=8),观察不同时段两组血浆一氧化氮和内皮素含量的变化。并在选择性逆向冠状静脉搭桥组经静脉桥在体灌注墨汁,石蜡包埋后切片观察。结果后降支结扎前,两组血浆一氧化氮和内皮素含量无显著性差异(P>0.05),但结扎后两组血浆一氧化氮和内皮素含量在不同时段比较差异有统计学意义(P<0.01)。显微镜下,经静脉桥灌注的墨汁能在心肌组织间均匀分布。结论选择性逆向冠状静脉搭桥能使心肌得到有效灌注,通过维持犬心肌缺血模型血浆一氧化氮及内皮素水平,从而减轻心肌内皮细胞损害,保护心脏功能。

Aim To investigate the effect of selective retrograde coronary venous bypass （SRCVB） on nitric oxide （NO） and endothelin （ET） of myocardial ischemia model in dogs. Methods 16 dogs were divided into ischemia group （n=8） and SRCVB group （n=8）. The levels of plasma NO and ET were measured in each group. In SRCVB group, 10 mL ink were utilized to perfuse the heart through SVG graft in vivo. Results The differences of NO and ET were not statistically significant before ligation of PDA in both groups （P〉0.05）, but changed significantly in SRCVB group after the ligation （P〈0.01）. The ink perfused retrogradely from graft distributed the myocardium tissue uniformly. Conclusions SRCVB could guarantee effective perfusion of myocardium and ameliorate the abnormal concentration of NO and ET in ischemic myocardium, decrease injury to endothelial cell and protect the heart.