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基质细胞衍生因子1α对大鼠骨髓源内皮祖细胞迁移的影响 被引量：6

Effects of Stromal Cell-Derived Factor-1α on Proliferation and Migration of Rat Bone Marrow Derived Endothelial Progenitor Cells
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摘要 目的观察基质细胞衍生因子1α对体外培养的大鼠骨髓源内皮祖细胞迁移的影响。方法微孔法从大鼠骨髓提取内皮祖细胞。免疫荧光鉴定血管内皮生长因子受体2和CD133,不同浓度基质细胞衍生因子1α处理内皮祖细胞后,采用Transwell迁移系统检测内皮祖细胞迁移能力。结果分离出的大鼠骨髓内皮祖细胞免疫荧光下血管内皮生长因子受体2+和CD133+双阳性,基质细胞衍生因子1α呈浓度依赖性促进内皮祖细胞迁移,对照组与基质细胞衍生因子1α各处理组比较差异均具显著性(P<0.05或P<0.01)。结论基质细胞衍生因子1α呈浓度依赖性促大鼠骨髓源内皮祖细胞迁移。 　Aim To study whether stromal cell-derived factor-1α （SDF-1α） have effects on endothelial progenitor cells （EPC） and its preliminary mechanism. Methods Bone marrow derived EPC were aquried by Micropore-method and characterized by immunofluorescence staining. The abilities of migration were detected by the methods of transwell migration after EPC treated with different concentrations of SDF-1α. Results SDF-1α promoted EPC biologic activity of migration in a concentration-dependent manner （P〈0.05 or P〈0.01）. Conclusion SDF-1α can improve migration of EPC.
作者 童中艺 王佐 姜志胜 宋砚明 周晓峰 田永凤
机构地区 常德职业技术学院基础医学部 南华大学心血管病研究所
出处 《中国动脉硬化杂志》 CAS CSCD 2007年第7期491-493,共3页 Chinese Journal of Arteriosclerosis
基金 中国博士后基金(2005038472)资助
关键词 病理学与病理生理学 基质细胞衍生因子1Α 内皮祖细胞 CXCR4 细胞迁移 Stromal Cell-Derived Factor-1α Endothelial Progenitor Cells CXCR4 Cell Migration
分类号 R363 [医药卫生—病理学]
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参考文献20

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二级参考文献24

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共引文献23

同被引文献95

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引证文献6

二级引证文献35

中国动脉硬化杂志
2007年 第7期

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