肝组织HBVcccDNA定量检测在慢性乙型肝炎诊断及治疗中的作用

Study of effect of treating and observing by quantitative assay of hepatitis B virus covalently closed circular DNA of hepatitis tissue in chronic hepatitis B patients

目的探讨肝组织HBVcccDNA定量在慢性乙型肝炎(CHB)诊断和治疗中的意义。方法使用荧光PCR检测仪,对85例CHB患者肝组织HBVcccDNA行定量检测,同时行肝组织及血清HBV-DNA定量检测。结果①85例CHB患者肝组织均检测到HBVcccDNA;②肝组织HBVcccDNA定量与肝组织HBV-DNA定量呈高度相关,与血清HBV-DNA定量亦呈正相关;③45例HBeAg阳性CHB患者应用拉米夫定治疗48周后,肝组织HBVc-ccDNA仍可维持一定水平(特别是血清HBV-DNA定量低于检测水平以下者)。结论肝组织HBVcccDNA定量检测,是评价HBV复制、感染持续、临床药物抗HBV疗效的可靠指标。

[ Objective] To explore the significance of liver tissue HBVcccDNA quantification in in the diagnosis and treatment of chronic hepatitis B（CHB）. [ Methods] Fluorescence PCR was used to detect HBVeecDNA quantitatively in liver biopsies and HBV-DNA in serum and liver biopsies of 85 CHB patients. [ Results] ①HBVcccDNA was detected in liver tissues of all CHB patients. ②There was a strong correlation between the HBVeecDNA and HBV-DNA in the hepatic tissue. There was also a positive correlation between the hepatic tissue HBVeeeDNA and HBV-DNA in the serum. ③45 HBeAg positive patients were treated for 48 weeks with Lamifudine, the hepatic tissues HBVeeeDNA still kept to a certain level （especially when level of serum HBV-DNA was low enough not to be detected）. [ Conclusion ] The quantitative detection of hepatic tissue HBVeeeDNA is a reliable indicator for evaluating HBV duplication, persistence of infection, and effect of antiviral drugs .