复发/难治AML患者sorcin基因表达及意义

Expression and clinical significance of sorcin gene in relapse or refractory AML patients

目的研究复发/难治急性髓细胞白血病(AML)患者骨髓可溶性耐药相关钙结合蛋白(sorcin)基因表达及意义。方法采用逆转录聚合酶链反应(RT-PCR)检测65例AML患者(观察组),27例非白血病患者和健康人(对照组)sorcin基因表达水平。结果观察组sorcin基因表达水平高于对照组(P<0.001),其中复发/难治者高于初诊者和完全缓解(CR)者;AML各亚型sorcin基因过度表达存在差异,以M5阳性表达率最高;观察组临床耐药者sorcin基因表达显著高于非临床耐药者(P<0.001),且CR率显著低于非临床耐药者(P<0.001)。结论sorcin基因过度表达与AML患者临床耐药密切相关,是影响预后的重要因素;sorcin可作为检测AML临床耐药和判断预后的指标之一。

[ Objective ] To study expression of soluble resistance-related calcium-binding protein （sorcin） gene and clinical significance in relapse or refractory Acute Myeloid Leukemia（AML） patients. [ Methods] A semi-quantitative reverse transcription polymerase chain reaction （RT-PCR） was used to investigate the transcription levels of the human sorcin gene in 65 AML patients and 27 controls, and analyzed the clinical significance of sorcin gene. [ Results] Sorein gene expression was significantly higher in AML patients than that in normal controls （ P 〈 0. 001 ）, and higher in relapsed/refractory AML patients than that in those newly diagnosed or complete remission（CR）. There had differences of sorcin gene expression in AML subtypes, and was highest in AML-M5 patients. Sorcin gene expression was significantly lower in non-resistant patients than that in resistant ones （P 〈 0. 001 ）. CR rate of non-resistant patients were higher than that of resistant ones （P 〈 0. 001 ）. [ Conclusion ] Sorein gene overexpression is significantly associated with clinical multidrug resistance and prognosis, and it is one of the indicators for drug resistance and predicting prognosis of AML patients.