甲氨基阿维菌素苯甲酸盐高效液相色谱分析方法研究

Study on quantitative analysis of emamectin benzoate by HPLC

研究用HPLC定量分析甲维盐的方法。色谱条件：25cm×4．6mm，5μm粒径的Kromasil—C18不锈钢柱；流动相甲醇-水（体积比为92：8）；流速1．0mL·min^-1；紫外检测器波长254nm。本方法在2．0—12μg进样范围内和峰高呈线性。相关系数值（R）为0．997。甲维盐分析的回收率为98％～102％，变异系数为4．39％。

A method of quantitative analysis for emamectin benzoate by HPLC has been studied. Conditions were as follows： column, 250mm×4.6nm stainless steel packed with Kromasil C18, 5m ;mobile phase, 8% H2O in methanol （V/V） ; flow rate, 1.0mL·min^-1 ; UV detector wavelength, 254nm. This analytical method is linear and reproducible over the range of 2 g to 12 g analyte. The correlation coefficient was 0. 997. Recovery of emamectin benzoate ranged from 98% to 102% ,with a coefficient of variation 4.39%.