摘要
目的探讨诱导分化剂苯乙酸(PA)对人结直肠癌细胞系HCT-8细胞周期及增殖的影响。方法应用MTT比色法,以1.0,2.0,3.0,4.0,5.0mmol/L的PA作用于体外培养的HCT-8细胞,分别于24,48,72h后对细胞增殖进行检测;流式细胞术分析细胞周期。结果随着PA浓度的增加(1~5mmol/L)或药物作用时间的延长(24~72h),肿瘤细胞生长抑制率明显增加。PA1~5mmol/L作用24h细胞生长抑制率为5.1%-24.3%,48h为16.7%-72.3%,72h为30.2%-93.4%。PA作用细胞72h后,G0/G1期比例显著下降,S期比例相对升高,组间差异显著(P〈0.05)。结论PA在诱导分化结直肠癌HCT-8细胞过程中,可诱导G1细胞周期阻滞,抑制细胞增殖。
Objective To investigate the effect of phenylacetate ( PA ) , a differentiation induce agent, on cell cycle and proliferation in colorectal carcinoma HCT-8 cell line. Methods HCT-8 cells were grown in the presence of PA (1.0, 2.0, 3.0, 4.0, and 5.0mmol/L) for24h, 48h and 72h, respectively. The cellular proliferation inhibitory ratio was evaluated by MTT assay. The different phases of the cell cycle were analyzed using flow cytometer. Results HCT-8 cells were treated by 1.0 - 5.0 mmol/L PA for 24 -72 h. With the increase of concentration of PA or the prolongation of the treatment time, the proliferation inhibitory ratio of the tumour cells increased notably by 5. 1% -24.3% when treated for 24h, 16.7% -72.3% when treated for 48 h, and 30.2 % - 93.4 % when treated for 72 h, respectively. When HCT-8 cells were treated with 5. 0 mmol/L PA for 72h, cell cycle analysis with flow cytometry showed that the percentage of HCT-8 cells in G0/G1 phase reduced significantly and S phase was relatively increased (P 〈0.05 ). Conclusions PA can arrest G, cell cycle and inhibit cell proliferation.
出处
《中国普通外科杂志》
CAS
CSCD
2007年第9期852-854,共3页
China Journal of General Surgery
基金
国家自然科学基金资助项目(39900152)
吉林省自然科学基金资助项目(20030417-02)
关键词
结直肠肿瘤
苯乙酸
细胞周期
增殖抑制
Colorectal Carcinoma
Phenylacetate
Cell Cycle
Proliferation Inhibition