苯乙酸对人结直肠癌细胞HCT-8的G_1细胞周期阻滞及增殖抑制作用

Phenylacetate inhibits cell proliferation via G_1 cell cycle arrest in the HCT-8 colorectal carcinoma cell line

目的探讨诱导分化剂苯乙酸（PA）对人结直肠癌细胞系HCT-8细胞周期及增殖的影响。方法应用MTT比色法，以1．0，2．0，3．0，4．0，5．0mmol／L的PA作用于体外培养的HCT-8细胞，分别于24，48，72h后对细胞增殖进行检测；流式细胞术分析细胞周期。结果随着PA浓度的增加（1～5mmol／L）或药物作用时间的延长（24～72h），肿瘤细胞生长抑制率明显增加。PA1～5mmol／L作用24h细胞生长抑制率为5．1％-24．3％，48h为16．7％-72．3％，72h为30．2％-93．4％。PA作用细胞72h后，G0／G1期比例显著下降，S期比例相对升高，组间差异显著（P〈0．05）。结论PA在诱导分化结直肠癌HCT-8细胞过程中，可诱导G1细胞周期阻滞，抑制细胞增殖。

Objective To investigate the effect of phenylacetate （ PA ） , a differentiation induce agent, on cell cycle and proliferation in colorectal carcinoma HCT-8 cell line. Methods HCT-8 cells were grown in the presence of PA （1.0, 2.0, 3.0, 4.0, and 5.0mmol/L） for24h, 48h and 72h, respectively. The cellular proliferation inhibitory ratio was evaluated by MTT assay. The different phases of the cell cycle were analyzed using flow cytometer. Results HCT-8 cells were treated by 1.0 - 5.0 mmol/L PA for 24 -72 h. With the increase of concentration of PA or the prolongation of the treatment time, the proliferation inhibitory ratio of the tumour cells increased notably by 5. 1% -24.3% when treated for 24h, 16.7% -72.3% when treated for 48 h, and 30.2 % - 93.4 % when treated for 72 h, respectively. When HCT-8 cells were treated with 5. 0 mmol/L PA for 72h, cell cycle analysis with flow cytometry showed that the percentage of HCT-8 cells in G0/G1 phase reduced significantly and S phase was relatively increased （P 〈0.05 ）. Conclusions PA can arrest G, cell cycle and inhibit cell proliferation.