摘要
以芦丁为标样,选择测定波长为502nm,用分光光度法测定鱼腥草叶总黄酮含量,结果表明:芦丁浓度在8.1248.72μg/mL范围内,吸光度和浓度呈良好的线性关系(R2=0.9998),测定的平均回收率为99.4%,精密度试验RSD=0.49%,重现性试验RSD=2.07%。该方法精密度高,结果稳定可靠。此外用纸色谱和显色反应对鱼腥草叶黄酮作了初步鉴别。
Total flavonoids content in the leaves of Houttuynia cordata Thunb was determined by Spectrophotometry at the wavelength of 502nm, with rutin as standard sample. The determination result showed： there was a linear relationship between the concentration of the rutin solution and absorption spectrum in the range of 8. 12 -48. 72μg g/mL （R^2 =0. 9998）. The average recovery rate of this method was 99. 4%, the RSD of precision test was 0. 49% and the RSD of reproducibility test was 2.07%. This method was good in precision and reproducibility. Furthermore, the preliminary identification of the leaves of Houttuynia cordata Thunb was done by paper chromatography and color reaction.
出处
《中国食品添加剂》
CAS
2007年第5期143-147,共5页
China Food Additives
关键词
鱼腥草叶
黄酮
分光光度法
鉴别
houttuynia cordata Thunb. leaves
flavonoids
spectrophotometry
identification
