急性脑梗死患者血清神经元特异性烯醇化酶水平变化及意义

Serum neuron-specific enolase concentrations changes and significace in patients with acute cerebral infarction

目的探讨急性脑梗死(ACI)患者血清神经元特异性烯醇化酶(NSE)动态变化的临床意义。方法采用酶联免疫吸附(ELISA)法检测40例ACI患者(观察组)血清NSE水平,并分析其与梗死体积、神经功能缺损程度[斯堪的那维亚卒中量表(SSS)评分分型]及梗死部位的关系。结果观察组血清NSE水平明显高于对照组(30例健康人),P<0.01;NSE水平与梗死灶体积和神经功能缺损程度呈正相关(P<0.01)。结论NSE是反映脑内神经元损伤或坏死的客观指标;可作为ACI早期诊断、判断病情转归和预后的综合指标。

[ Objective ] To evaluate the dynamic changes and clinical significance of neuron-specific enolase （NSE） concentrations in serum from patients with acute cerebral infarction （ACI）. [ Methods ] The serum levels of NSE were determined in 40 patients with ACI and 30 control subjects by enzyme-linked immunosorbent assay （ELISA）. The neurological status was evaluated by Scandinavia Stroke Scale （SSS） after onset of ACI, and the CT was also performed to calculate the infarct volume and to locate the infarct site . Statistical analyses were performed by. using a SPSS 11.5 statistical package. [ Results ] Serum concentrations of NSE increased within 48 hours after ACI in ACI group ; peaked at 3-5 day and were higher than those of the control group （ P 〈 0.01 ）. There was a positive correlation beween NSE concentrations in serum and infarct volume, the neurological deficit degree（ P 〈0.01 ）. [ Conclusion] NSE can be used as a objective index of neuroal injury or necrosis, and it can be used as a proper peripheral biochemical marker of brain-tissue damage. Its levels in serum may be served as a comprehensive index of early diagnosis, evaluation of seriousness and prognosis in ACI.