高效阴离子交换色谱分离-脉冲安培检测法测定烟草料液中的糖、糖醇和醇类化合物

Determination of Sugars and Sugar Alcohols in Tobacco Feed Liquids by High Performance Anion-Exchange and Pulsed Amperometric Detection

建立了高效阴离子交换色谱分离-脉冲安培检测法测定烟草料液中的糖、糖醇以及醇类化合物的方法。以NaOH为淋洗液，在CarboPac MA1阴离子交换柱上等度分离了肌醇、甘油、丙二醇、半乳糖醇、木糖醇、山梨醇、甘露醇、葡萄糖、果糖、蔗糖、甘露糖和半乳糖等12种化合物。对影响分离和检测的条件进行了优化，并在此优化条件下分析了烟草料液中的糖、糖醇以及醇类化合物。12种化合物的检出限为2．0～216μg／L（25．0μL进样，以3倍信噪比计算检出限）。12种化合物浓度为1～5mg／L的标准溶液连续7次进样的RSD为0．7％～4．3％。方法对烟草料液中12种化合物的加标回收率为80％～108％。方法灵敏、高效、简便、快捷。

A chromatographic method was developed for the determination of inositol, galactitol, glycerol, xylitol, propylene glycol, sorbitol, mannitol, glucose, fructose, sucrose, mannose and galactose in tobacco feed liquid samples using high-performance anion-exchange chromatography coupled with pulsed amperometric detection. The separation was carried out on CarboPac MA1 anion-exchange column using NaOH eluent. Moreover, the conditions affecting the separation and detection were optimized. The detection limits ranged from 2.0 to 7.6 μg/L and the linear range of 0.1 to 50 mg/L was obtained for inositol, galactitol, glycerol, xylitol. The detection limits ranged from 9.0 to 11.9 μg/L and the linear range of 1 to 50 mg/L was obtained for propylene glycol, sorbitol, mannitol, glucose, mannose and galactose. For fructose and sucrose, the detection limits were 102 μg/L, 216μg/L respectively and linear range was 2 -100 mg/L （25.0 μL injection, S/N = 3 ）. The RSD of the twelve analytes （1.0 -5.0 mg/L） was 0.7% -4.3% （n =7）. In addition, the spiked recoveries of the twelve analytes in tobacco feed liquid were 80% - 108%.