一种四步法提取分离白念珠菌总蛋白质方法的建立

Establishment of a "Four-step method" for extraction and separation of Candida albicans total proteins

目的:建立白念珠菌总蛋白质分步提取分离的方法。方法:利用四种溶解能力不同的溶液分步提取白念珠菌总蛋白,定量后,分别进行一维等电聚焦分离和二维聚丙烯酰胺凝胶电泳分离。结果:成功分步提取获得白念珠菌不同溶解度的蛋白,得到各步提取蛋白的二维凝胶电泳图谱。结论:四步法提取白念珠菌总蛋白质是研究白念珠菌蛋白质组的有效方法,为进一步研究白念珠菌耐药机制以及抗真菌药物作用机制奠定基础。

Objective：To introduce a ＂Four-step method＂ for extraction and separation of Candida albicans total proteins. Methods： Proteins of C. albicans were extracted step-by-step with 4 kinds of solutions with different solubilities. After quantification, the protein samples were separated by isoelectric focusing electrophoresis and then by SDS-PAGE. Results： Proteins with different solubilities were successfully extracted step-by-step from C. albicans and were separated by two-dimensional gel electrophoresis. Conclusion： The ＂Four-step method＂ for extraction of C. albicans proteins is an effective approach to study C. albicans membrane proteome and lays a foundation for further investigation of the mechanisms of antifungal agents and drug resistance in C. albicans.