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Bay 11-7082增强Fas介导的HL-60细胞凋亡作用 被引量：1

Enhancement of Fas-mediated Apoptosis in Leukemic Cell Line HL-60 by Bay 11-7082
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摘要 本研究观察NF-κB抑制剂Bay 11-7082对HL-60细胞Fas/FasL系统的作用及对Fas介导的HL-60细胞凋亡的影响。用RT-PCR和流式细胞术检测Bay 11-7082干预后HL-60细胞Fas、FasL和XIAP的mRNA和蛋白表达水平的变化;用ELISA方法检测Bay 11-7082干预前后sFasL水平的变化;流式细胞术检测Bay 11-7082干预前后CH-11(活性Fas抗体)诱导HL-60细胞凋亡的变化。结果表明:用Bay 11-7082干预后,HL-60细胞FasL和XIAP的mRNA和蛋白水平较对照组明显下降,差异具有显著性(p<0.05),Fas的mRNA、蛋白水平的变化和sFasL水平的变化无显著的差异(p>0.05);Bay 11-7082干预后HL-60细胞对CH-11诱导细胞凋亡敏感性显著增强。结论:Bay 11-7082可能通过下调FasL和XIAP水平增强HL-60细胞Fas介导的凋亡。 The aim of study was to explore the effects of NF-κB inhibitor Bay 11-7082 on Fas/FasL system and Fasmediated apoptosis in HL-60 cells. The mRNA and protein expression levels of Fas, FasL and XIAP after treatment with Bay 11-7082 were detected by RT-PCR and FCM respectively. The level of sFasL was detected by ELISA before and after treatment with Bay 11-7082; apoptosis was detected by FCM before and after treatment with Bay 11-7082. The results showed that after treating HL-60 cells with Bay 11-7082, the mRNA and protein levels of FasL and XIAP were lower than that of controls, the difference was significant by statistic analysis （p 〈 0.05 ）. Neither the mRNA and protein levels of Fas, nor the level of sFasL changed significantly （p 〉 0. 05 ）. Apoptotic rate of HL-60 cells treated with Bay 11 - 7082 was significantly higher as compared with controls （p 〈 0. 05 ）. It is concluded that Bayl 1-7082 can enhance Fasmediated apoptosis in HL-60 cells by down-regulation of FasL and XIAP levels.
作者 王利 刘凌波 李蕾 邹萍
机构地区 华中科技大学同济医学院附属协和医院血液病研究所
出处 《中国实验血液学杂志》 CAS CSCD 2007年第5期941-945,共5页 Journal of Experimental Hematology
关键词 BAY 11-7082 FAS/FASL系统 HL-60 NF—κB Bay 11-7082 Fas/FasL system HL-60 NF-κB
分类号 R733.7 [医药卫生—肿瘤]
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参考文献11

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  • 8Zhu B, Liu Z, Wang P, et al. A nuclear factor-kappaB inhibitor BAY11-7082 inhibits interactions between human endothefial cells, T cells, and monocytes[J]. Transplant Proc, 2008, 40(8): 2724-2728.
  • 9Mendez-Samperio P, Trejo A, Perez A. Mycobacterium boris bacil- lus Calmette-Guerin induces CCL5 secretion via the Toll-like re- ceptor 2-NF-kappaB and-Jun N-terminal kinase signaling path- ways[J]. Cllin Vaccine Immunol, 2008, 15(2): 277-283.
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中国实验血液学杂志
2007年 第5期

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