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套层细胞淋巴瘤细胞系中染色体13q31-q32上miR-17-92基因簇的DNA序列研究 被引量：1

DNA Sequencing of miR-17-92 Cluster at Chromosome 13q31-q32 in Mantel Cell Lymphoma Cell Lines
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摘要 为了研究B细胞淋巴瘤的染色体13q31-q32上miR-17-92基因簇的特点,观察B细胞淋巴瘤中miR-17-92基因簇在基因组DNA水平上是否存在改变,及其对miR-17-92基因簇的表达和与淋巴瘤发生关系的影响。应用PCR和DNA序列测定技术检测Rec1、G519和Z138三个套细胞淋巴瘤(mantel cell lymphoma,MCL)细胞系中染色体13q31-q32上miR-17-92基因簇。结果表明,3个MCL细胞系的miR-17-92基因簇DNA序列与正常人的序列比对完全一致。由此可以认为,MCL细胞系中miR-17-92基因簇在DNA序列上没有异常改变,不是miR-17-92基因簇的过度表达原因。 This study was aimed to explore the characteristics of miR-17-92 cluster at chromosome 13q31-q32 in B cell malignant lymphoma and to investigate the changes of miR-17-92 chuster in B cell lymphoma at genome DNA level and its influeuce on expression of miR-17-92 cluster and relation with lymphoma occurence, PCR and DNA sequencing were used to detect miR-17-92 cluster at chromosome 13q31-q32 in mantel cell lymphoma （MCL） cell lines Recl, G519 and Z138, The results showed that DNA sequence of miR-17-92 cluster at chromosome 13q31-q32 in MCL cell lines were normal, It is concluded that there is no abnormal change in DNA sequence of miR-17-92 cluster which is not the mechanism for miR-17-92 cluster overexpression in mantel cell lymphoma cell lines.
作者 徐卫 李建勇 沈秋丹 李丽 于慧
机构地区 南京医科大学第一附属医院
出处 《中国实验血液学杂志》 CAS CSCD 2007年第5期986-988,共3页 Journal of Experimental Hematology
基金 "江苏省医学领军人才"项目资助 江苏省自然基金资助项目 编号BK2007249
关键词 MIRNA miR-17—92基因簇 淋巴瘤 miRNA miR-17-92 cluster lymphoma
分类号 R733.1 [医药卫生—肿瘤]
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参考文献9

  • 1徐卫,李建勇,陆凤翔.微小RNA及其在恶性淋巴增殖性疾病中作用的研究进展[J].中国实验血液学杂志,2006,14(4):840-844. 被引量：4
  • 2Ota A, Tagawa H, Karnan S, et al. Identification and characterization of a novel gene, C13orf25, as a target for 13q31-q32 amplification in malignant lymphoma. Cancer Res,2004;64:3087-3095
  • 3Krek A, Grun D, Poy MN, et al. Combinatorial microRNA target predictions. Nat Genet, 2005; 37: 495-500
  • 4Chen PY, Meister G. microRNA-guided posttranscriptional gene regulation. Biol Chem, 2005; 386:1205-1218
  • 5Thomson JM, Newman M, Parker JS, et al. Extensive post-transcriptional regulation of microRNAs and its implications for cancer. Genes Dev, 2006; 20:2202-2207
  • 6Zhang B, Pan X, Cobb GP, et al. microRNAs as oncogenes and tumor suppressors. Dev Biol, 2007; 302:1-12
  • 7Kent OA, Mendell JT. A small piece in the cancer puzzle: microRNAs as tumor suppressors and oncogenes. Oncogene, 2006; 25:6188-6196
  • 8He L, Thomson JM, Hemann MT, et al. A microRNA polycistron as a potential human oncogene. Nature, 2005; 435（7043）:828-833
  • 9O′Donnell KA, Wentzel EA, Zeller KI, et al. c-Myc-regulated microRNAs modulate E2F1 expression. Nature, 2005; 435（7043）:839-843

二级参考文献38

  • 1Lee RC,Feinbaum RL,Ambros V.The C.elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14 RNA.Cell,1993;75:843-854
  • 2Reinhart BJ,Slack FJ,Basson M,et al.The 21-nucleotide let-7 RNA regulates developmental timing in Caenorhabditis elegans.Nature,2000;403:901-906
  • 3Pasquinelli AE,Reinhart BJ,Slack F,et al.Conservation of the sequence and temporal expression of let-7 heterochronic regulatory RNA.Nature,2000;408:86-89
  • 4Lee RC,Ambros V.An extensive class of small RNAs in Caenorhabditis elegans.Science,2001;294:862-864
  • 5Ambros V,Bartel B,Bartel DP,et al.A uniform system for microRNA annotation.RNA,2003;9:277-279
  • 6Ruvkun G.Molecular biology.Glimpses of a tiny RNA world.Science,2001;294(5543):797-799
  • 7Lau NC,LimL P,Weinstein EG,et al.An abundant class of tiny RNAs with probable regulatory roles in Caenorhabditis elegans.Science,2001;294(5543):858-862
  • 8Sun Y,Koo S,White N,et al.Development of a micro-array to detect human and mouse microRNAs and characterization of expression in human organs.Nucleic Acids Res,2004;32:e188
  • 9Lagos-Quintata M,Rauhut R,Lendeckel W,et al.Identification of novel genes coding for small expressed RNAs.Science,2001;294(5543):853-858
  • 10Reinhart BJ,Weinstein EG,Rhoades MW,et al.MicroRNAs in plants.Genes Dev,2002;16:1616-1626

共引文献3

同被引文献30

  • 1王晓薇.肿瘤细胞微环境对癌症转移力的影响[J].中州大学学报,2010,27(1):120-123. 被引量：1
  • 2张晋琳.中枢神经系统淋巴瘤研究进展[J].实用医院临床杂志,2007,4(6):25-28. 被引量：4
  • 3Tali Siegal, Neta Goldschmidt. CNS prophylaxis in diffuse large B-cell lymphoma: If, when, how and for whom?[J]. Blood Reviews, 2012, 26:97-106.
  • 4Clozel T, Yang S, Elstrom RL, et al. Mechanism-based epigenetic them osensitization therapy of diffuse large B-cell lymphoma [J]. Cancer Diseov, 2013, 3(9): 1002-1019.
  • 5Claire Galand, Sabrina Dormou, Thierry Jo Molina, et al. Influence of tumor location on the composition of immune infiltrate and its impact on patient survival. Lessons from DCBCL and animal models [J]. Front lmmunol, 2012, 3:98.
  • 6Chang JE, Seo S, Kim KM et al.Rituximab and CHOP chemotherapy plus GM-CSF for previously untreated diffuse large B-cell lymphoma in the elderly: a Wisconsin ontology network study [J]. Clin Lyrnphoma Myeloma Leuk, 2010, 10(5): 379-384.
  • 7Wei Ye, Liu Zhi-bin, Wang Li-chao, et al. Tight junction in blood-brain barrier: an overview of structure, regulation, and regulator substances [J]. CNS Neuroscienee & Therapeutics, 2012, 8 (18): 609-615.
  • 8Roehle A, Hoeleg KP, Repsilber D, et al. MicroRNA signatures characterize diffuse large B-cell lymphomas and follicular lyrnphomas[J]. British Journal of Haematology, 2008, 142(5): 732.
  • 9Lawrie CH, Gal S, Dunlop HM, et al. Detection of elevated levels of turnout associated microRNAs in serum of patients with diffuse large B cell lymphoma [J]. British journal of haematology, 2008, 141: 672- 675.
  • 10Chart YC, Banerjee J, Choi SY, et al. miR 210: the master hypoxamir [J]. Microcirculation, 2012, 19:215-223.

引证文献1

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中国实验血液学杂志
2007年 第5期

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