骨髓间充质干细胞输注对再生障碍性贫血模型小鼠骨髓造血功能的影响

Effect of Bone Marrow Mesenchymal Stem Cell Infusion on Hemato-poiesis in Mice with Aplastic Anemia

本研究探讨人源骨髓间充质干细胞(MSC)输注对免疫介导性再生障碍性贫血模型小鼠骨髓造血功能及生存率的影响.采用雌性BALB/c小鼠30只,参照姚军等方法建立免疫介导性再生障碍性贫血模型,实验分模型组、MSC组及照射组。MSC组于制模后3天从小鼠尾静脉1次性输注人骨髓MSC1×106,观察各组小鼠外周血象变化、第28天存活率、骨髓有核细胞数量、造血集落生成和骨髓病理学特征。结果显示:于制模后第7天,模型组外周血WBC显著低于MSC组和照射组,分别为(0.65±0.05)×109/L,(2.45±0.71)×109/L,(2.30±0.33)×109/L;第10天3组均表现为全血细胞减少,第14天模型组血象指标仍持续降低,而MSC组及照射组血象指标开始回升,至28天3组小鼠存活率分别为18.2%、80%和100%,MSC组小鼠存活率显著高于模型组(p<0.01)。MSC组小鼠单侧股骨有核细胞数量、造血祖细胞集落生成数量均显著高于模型组,而与照射组结果一致。结论:MSC输注可减轻再生障碍性贫血模型小鼠骨髓造血衰竭程度,提高存活率。

To explore the effects of infusion with human mesenchymal stem cells from bone marrow on bone marrow hematopoietic function and survival in mice with aplastic anemia （AA）, immuno-mediated aplastic anemia mice model was established according to Yao＇s method. Thirty BALB/c female mice were divided into AA model group, MSC group and radiation group. In MSC group, 1 ×10^6 MSC was infused by intravenous injection at 3 days after establishment of model. The changes of blood count, the number of nuclear cells in a single thigh-bone, colony-forming units（ CFU）, pathological features of bone marrow and survival rate of mice were observed in all groups. The results showed that the number of white blood cells in peripheral blood of AA model group at day 7 after establishment of model was （ 0.65 ± 0.05 ） ×10^9/L , which was significantly lower than those in MSC group and radiation alone group, respectively. Pancytopenia was found in all three groups at day 10. At day 14 the pancytopenia was continued in AA model group while it was less severe in MSC and radiation groups. In MSC group the number of nucleated cells in single thigh bone and pathological slice as well as CFU-GM were significantly higher than those in AA model group, respectively. The survival rate of mice in MSC group was 80.0% which was higher than that of group in AA model （ 18.2%, p 〈 0.01 ）. There were no significant differences between MSC group and radiation alone group in bone marrow hematopoiesis and survival rate. It is concluded that MSC infusion reduces the degree of bone marrow failure and improve survival of immuno-mediated AA mice model.