两种CpG ODN佐剂对小鼠白血病局灶瘤的抗瘤作用研究

Anti-tumor Effect of Two Adjuvants of CpG ODN on Leukemic Tumor in Mouse Models

为了比较免疫佐剂CpG ODN1826和CpG ODN2006在白血病局灶肿瘤动物模型中的抗瘤作用和毒副作用,筛选合适的免疫佐剂,建立了急性淋巴细胞白血病局灶肿瘤动物模型,在不同时间用灭活的L1210细胞和不同免疫佐剂给DBA/2小鼠皮下注射,观察小鼠一般状况、成瘤率、瘤块生长情况,了解瘤块病理情况。结果表明:灭活细胞加CpGODN1826瘤苗可以降低小鼠成瘤率,成瘤小鼠的瘤块生长缓慢,瘤块内部肿瘤细胞坏死明显,但生存期无明显延长;CpGODN2006组小鼠成瘤率不但降低,瘤块缩小,而且生存期也明显延长,已生长的肿瘤消退。结论:应用CpGODN2006瘤苗可以增强白血病局灶肿瘤小鼠的抗瘤作用,使生存期明显地延长,无明显的毒副作用。

To investigate the anti-tumor and side effect of CpG ODN 1826 and CpG ODN2006 as an adjuvants on leukemic tumor in mouse-models, an acute lymphocytic leukemic tumor in mouse model was established, then inoculated inactivated L1210 cells alone or with different vaccine adjuvants were injected subcutaneously into each DBA/2 model mouse at different times. The activities of mice, the tumor formation rate and the growth status of leukemic tumor were observed. The tumor was examined by pathologic section. The results showed that the vaccine of inactivated L1210 cells and CpG ODN 1826 could decrease the leukemic tumor formation rate, slow down the growth of leukemic tumor mass in mice and obviously cause necrosis of tumor cells, but it could not prolong the life spans of the tumor-burden mice ; while CpG ODN2006 could not only decrease the tumor formation rate, slow down the growth of tumor mass in mice and result in obvious necrosis of tumor cells, but also could eliminate the existing tumor mass in mice, and prolong the life spans of the tumor-burden mice. It is concluded that using CpG ODN2006 as an adjuvant enhances the anti-tumor effect against the leukemic tumor, prolong the life span of tumor-burden mice without obvious side effect.