间歇训练对负荷游泳力竭运动后即刻及恢复期大鼠心肌脂质过氧化及钙离子代谢的影响

Effects of Intermittent Training on Lipid Peroxidation and Calcium Ion Metabolism of Rat Myocardium Immediately after Exhaustive Swimming Exercise and during Recovery Period

方法:将大鼠随机分为两大组:对照组(A组)和间歇训练保护组(B组),A组常规喂养6周,B组间歇训练6周,6周后,除安静组外,其它组进行一次性力竭游泳训练,分别在即刻、6小时和24小时取材。测定血清CK及心肌组织SOD、GSH-PX、MDA、Ca2+。结果:B组力竭后3个时相点较A组SOD、GSH-Px活性非常显著性升高;A、B组力竭后3个时相点与安静时比MDA水平均非常显著地升高,A组在6小时最高,但B组力竭后3个时相点升高幅度均较A组低,在6小时有非常显著性差异:A、B组力竭后2个时相点与安静时比Ca2+水平均非常显著地升高,但B组力竭后3个时相点升高幅度均较A组低:在6小时B组CK非常显著地低于A组。结论:间歇训练作为一种运动预处理可显著提高力竭运动后的心肌组织SOD、GSH—PX活性,减轻脂质过氧化程度,保护细胞膜的完整性,改善钙离子代谢。

Methods： randomly divide into two groups, namely the control group （A group） and the protection group （ B group）. A group was fed for 6 weeks routinely and B group were given intermittent training for 6 weeks. Six weeks later, all groups except the no -exercise control group took an exhaustive swimming training, and were evaluated serum CK and SOD, GSH - Px, MDA, Ca^2 ＋ of myocardium at immediate time, and 6 and 24 hours later. Result： compared to group A, activity of SOD, GSH - Px in group B increased evidently at the three time phases. Contrast with the no - exercise control group, MDA obviouly rised after exhaust in both group A and B in the three time phases, and group A was highest after 6 hours. But its increase after exhaust was significantly smaller in group B than that in group A, and an obvious difference appeared after 6 hours. Ca^ ＋also increased greatly after exhaust in both group A and B in the three time phases, comparing with the no -exercise control group, but the increase in group B was smaller than that in group A. CK in group B was obviously lower than that in group A after 6 hours. Conclusion： intermittent training as exercise preconditioning can greatly increase the activity of SOD, GSH - Px after exhaustive exercise, decrease the degree of lipid peroxidation, protect the integrity of cell membrane and improve calcium ion metabolism as well.