螺旋藻多糖硫酸酯化物的物化性质、结构鉴定及初步药理活性研究

Study on the Physical and Chemical Properties and Pharmacological Activities of Sulfated Polysaccharide from Spirulina Platensis

目的:制备螺旋藻多糖的硫酸酯化物(SPSP),初步了解其物化性质、结构及药理活性。方法:采用氯磺酸-吡啶法合成硫酸酯化物,通过电泳、紫外和红外光谱及核磁共振等方法进行检测和鉴定,并进行了体内外抗肿瘤和抗病毒的初步试验研究。结果:得到分子质量约为10000的多糖,硫酸化修饰后多糖的最高取代度可达1.0086。在体外,当SPSP的浓度为400mg/L时,其对Lovo、KB细胞的抑制率最高,分别为48.33%和35.98%,但对SGC7901,S180细胞没有明显的抑制作用;对荷瘤小鼠亦无作用。SPSP在浓度为0.0063mg/mL时能很好地抑制甲型流感病毒引起的细胞病变。结论:所得化合物确为SPSP,且其对某些肿瘤和病毒具有抑制活性。

Objective： To obtain and prepare sulfated polysaccharide from Spirulinaplatensis （PSP） and to find out its the physical and chemical properties, structure and basic pharmacological activities . Methods： The sulfated polysaccharide was synthesized by chlorosulfonic-pyridine method. Its structure was identificated by electrophoresis, molecular weight determination, UV, IR and NMR. Its activities were determined through anti-tumor and anti-virus studies in vitro and in vivo. Results： The average relative molecular mass of SPSP is about 10 000 D and its degree of substitution is 1.008 6. SPSP of 400μg/mL has the highest inhibitory activity of 48.33% and 35.98% on Lovo and KB, respectively, but it has no significant inhibitory activity on SGC7901 and S180 in vitro and can not inhibit S180 in vivo. It shows a strong ability to inhibit CPE caused by FM1 at 0.006 3 mg/mL. Conculsion： The compound obtained by this systhesis is SPSP and it has a significant inhibiting effect on some kinds of cancer cells and viruses at lower concentrations.