摘要
目的研究兔尿道损伤后尿道瘢痕组织中TGF-β1表达水平。方法选择成年健康雄性兔30只,其中6只作为正常对照,另24只作创伤模型之用,按建模后不同观察时段10天、1、3、6个月分为4组;采用west-ern-blot检测尿道瘢痕组织中TGF-β1蛋白水平。结果正常对照组TGF-β1蛋白表达水平(0.72±0.12)ng/ml;创伤后第10天、1、3、6个月的TGF-β1蛋白表达水平分别为(4.8±0.3)ng/ml、(11.5±0.5)ng/ml、(12.1±0.7)ng/ml、(5.6±0.6)ng/ml,尿道损伤后瘢痕组织中TGF-β1蛋白表达水平高于正常对照组。结论TGF-β1蛋白表达水平在兔的尿道创伤修复后的瘢痕组织中高水平表达,TGF-β1蛋白可能在尿道损伤后瘢痕形成中起重要作用。
Objective To study the expression of TGF-β1 in the rabbit urethral scar tissue after urethral injury. Methods Thirty adult healthy male rabbits were used in the study ： the control group included 6 ; the others were divided into 4 groups by the different observed time： 10 days, 1 month, 3months and 6months after injury. The expression level of TGF-β1 protein in the rabbit urethral scar tissue was detected by the western-blot. Results The expression level of TGF-β1 in normal group was 0. 72 ±0. 12 ng/ml; and 4. 8 ±0. 3 ng/ml, 11.5 ±0. 5 ng/ml, 12. 1 ±0. 7 ng/ml, 5.6 ±0. 6 ng/ml in the 10 days, lmonth, 3months, 6months after urethral injury. The expression level of TGF-β1 after urethral injury was significantly higher than normal control group. Conclusion The TGF-β1 protein was highly expressed in the rabbit urethral scar tissue after urethral injury and TGF-β1 may play an important role in the formation of scar after uretharal injury.
出处
《广州医药》
2007年第5期49-50,共2页
Guangzhou Medical Journal
