红姜花的组织培养和快繁技术研究

Study on Alpinia purpurata in Vitro and Its Rapid Propagation

以红姜花花序抽生的腋芽诱导丛生苗和愈伤组织以及植株再生,结果表明:外植体诱导培养基以MS+6-BA2.0mg·L-1+NAA2.0mg·L-1为宜,不定苗的诱导率可达158.3%;丛生苗继代增殖培养基以MS+6-BA1.0mg·L-1+NAA1.0mg·L-1为宜,增殖倍数可达5.6倍;愈伤组织诱导培养基以MS+6-BA6.0mg·L-1+NAA0.1mg·L-1;植株再生和丛生苗增殖培养基以MS+6-BA1.0mg·L-1+NAA1.0mg·L-1;生根诱导以1/2MS+NAA0.5mg·L-1或1/2MS+NAA0.5mg·L-1+6-BA0.2mg·L-1两种培养基为宜,生根诱导率可达100%。

Axillary bud induction of cluster bud and callus and plant regeneration from inflorescence of Alpiniapurpurata was made. The result showed that the optimum medium for explant induction was MS＋6-BA2.0mg/L＋NAA2.0mg/L; The proliferation can reach to 5 to 6 times while in the medium of MS＋6-BA1.0mg/L＋NAA1.0mg/L for inherit multiplication, and MS＋6-BA6.0mg/L＋NAA0. 1mg/L far callus induction. Besides, in the medium of MS＋6-BA1.0mg/L＋NAA 1.0mg/L for plant regeneration and cluster proliferation was the optimum one. The roofing rate can reach the highest （100%） while in both medium of 1/2MS＋NAA0.Smg/L and 1/2MS＋NAA0.Smg/L＋6-BA0.2mg/L for induction of roots.