葛根素对糖尿病P-选择素及主动脉血管细胞粘附分子mRNA表达调节的实验研究

Study of regulation of Puerarin on blood P-selectin and the expression of aorta VCAM mRNA in diabetes

目的观察葛根素对糖尿病大鼠P-选择素及主动脉血管细胞粘附分子(VCAM)mRNA表达的调节。方法利用链脲佐菌素腹腔注射法诱导建立1型糖尿病大鼠模型,将实验用SD大鼠随机分正常对照组、糖尿病组、葛根素3个剂量组[20、40和80mg/(kg.d)ip]。处理16周,观察处理后大鼠的胆固醇、低密度脂蛋白、高密度脂蛋白、P-选择素、糖化低密度脂蛋白、氧化低密度脂蛋白,分离主动脉,HE染色观察主动脉病理形态改变及原位杂交检测主动脉内膜VCAMmRNA表达。结果(1)造模4组大鼠均出现血脂异常及主动脉病理形态改变。(2)葛根素能降低糖尿病大鼠的P-选择素、胆固醇、低密度脂蛋白、氧化低密度脂蛋白(P<0.05),升高高密度脂蛋白(P<0.05);葛根素降低主动脉VCAMmRNA(P<0.05)表达,且呈一定的剂量-反应关系。结论葛根素通过降低黏附分子的表达,起到确切的主动脉保护作用。

Objective To observe the regulation of Puerarin on blood P-selectin and expression of aorta vascular cell adhesion molecule （VCAM） in diabetic rats induced by Streptozotocin. Methods Diabetic rats were induced by intrapefitoneal injection of STZ. Rats were divided into normal control group, model group, and three Puerarin groups （20, 40 and 80mg/kg,ip） for 16 weeks. After the treatment, its cholesterol, low density lipoprotein, high density lipoprotein, P-selectin, oxidative low density lipoprotein and glycosylated low density lipoprotein. Alteration of tissue morphology via H. E staining was observed after the aorta was taken out from the rats executed by narcosis. VCAM mRNA were determined by in situ hybridization analysis. Results （1）Diabetes mellitus and aorta lesion occurred in the four model groups. （2） Puerarin could decrease the levels of cholesterol（ P 〈 0.05） , low density lipoprotein（ P 〈 0.05）, P-selectin（ P 〈 0.05） , oxidative low density lipoprotein, and glycosylated low density（ P 〈 0.05）, lipoprotein increase high density lipoprotein （ P 〈 0.05）. The gene expression of VCAM in the aorta were significantly inhibited, and the action of Puerarin showed some dose reaction relationship at the same time （ P 〈 0.05）. Conclusion Puerarin have a certain in preventing aorta by inhibiting expression of adhesion molecule.