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丙烯酰胺致小鼠睾丸生殖细胞DNA损伤与细胞增殖影响研究 被引量：4

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摘要 目的了解丙烯酰胺致生殖毒性的DNA损伤机制。方法昆明种小鼠经丙烯酰胺(20、40和60mg/kgbw)慢性染毒8周后,利用单细胞凝胶电泳研究睾丸生殖细胞的DNA损伤;四甲基偶氮噻唑蓝(MTT)法研究细胞存活率。结果阴性对照组细胞呈正常的圆形,阳性对照组的睾丸细胞及丙烯酰胺染毒8周后的各组受损细胞增加,40mg/kg和60mg/kg组的细胞拖尾率及尾长显著高于阴性对照(P<0.05);不同剂量丙烯酰胺组的睾丸细胞存活率均有所下降,其中40mg/kg和60mg/kg组的细胞存活率显著低于阴性对照组(P<0.05)。结论丙烯酰胺对生殖细胞有明确的DNA损伤作用,且在一定剂量范围内有蓄积性。
作者 郭彩华 卢珍华 毛德倩 陈昭华 张希春
机构地区 集美大学生物工程学院 中国疾病预防控制中心营养与食品安全所
出处 《卫生研究》 CAS CSCD 北大核心 2007年第5期610-611,共2页 Journal of Hygiene Research
基金 集美大学中青年创新团队专项基金(No.2006A003)
关键词 丙烯酰胺 生殖细胞 单细胞凝胶电泳 睾丸 DNA损伤
分类号 R734.12 [医药卫生—肿瘤] R994 [医药卫生—毒理学]
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参考文献4

  • 1宋雁,李宁.食品中丙烯酰胺对健康的影响[J].卫生研究,2005,34(2):241-243. 被引量：28
  • 2GHANAYEM B I, WITT K L, KISSLING G E, et al. Absence of acrylAAide-induced genotoxicity in CYP2E1-null mice: evidence consislent wilh a glycidamide-mediated effect[J]. Mut Res,2005,578(1-2) :284-297.
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二级参考文献15

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共引文献27

同被引文献94

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二级引证文献13

卫生研究
2007年 第5期

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