海洋红酵母产类胡萝卜素的研究

THE STUDY ON CAROTENOIDS PRODUCTION BY RHODOTORULA OF MARINE

从海洋中筛选出1株产类胡萝卜素能力较强的红酵母RY-8。研究了不同碳源、氮源、装液量和pH值对菌株RY-8生物量和产类胡萝卜素的影响,并通过正交试验优化其产类胡萝卜素的发酵条件。结果表明,红酵母菌株RY-8产类胡萝卜素的最佳发酵条件为:葡萄糖25g/L,酵母膏10g/L,装液量60mL/250mL,pH值6.0。在此优化条件下,红酵母RY-8的生物量和类胡萝卜素产量分别为48.8g/L和4.76mg/L,依次比初始产量提高了20.8%和38.4%。

The Rhodotorula strain RY-8 with high carotenoids yields was screened from marine.The effect of carbon source, nitrogen source, volumes and pH on biomass and carotenoids yields of RY-8 were studied.The optimal conditions for carotenoids fermentation were studied with orthogonal test.The results showed that the optimal conditions was as follows：glucose 25 g/L, yeast extract 10 g/L, volumes 60 mL/250 mL, pH6.0. The biomass and carotenoids yields of strain RY-8 could be 48.8 g/L and 4.76 mg/L in optimal medium, 20.8 % and 38.4 % were higher than the first separation yields.