高效液相色谱法测定蜜饯中食用合成色素

METHOD FOR DETERMINATION OF SYNTHETIC COLORS IN PRESERVED FRUIT

建立了蜜饯中食用合成色素(柠檬黄、苋菜红、胭脂红、日落黄、亮蓝)的高效液相色谱测定方法,采用C18(4.6mm×200mm,5μm)色谱柱,甲醇和乙酸铵为流动相,流速为1.0mL/min,柱温30℃,梯度洗脱程序为:甲醇+0.02mol/L乙酸铵,6%￣40%甲醇,2.8%/min;40%￣60%甲醇,2.5%/min;60%甲醇,3min;6%甲醇,7min;检测波长:0min￣5.5min,430nm;5.51min￣8.0min,520nm;8.01min￣10.0min,510nm;10.01min￣13min,480nm;13.01min￣20min,600nm。确定了蜜饯中合成色素的前处理方法。本方法具有灵敏度高,干扰少,色素回收率高的优点。

Method for determination of synthetic colors （Tartrazine, Amaranth, Ponceau 4R, Sunset yellow, Brilliant blue ） in preserved fruit was developed. It was performed on a C18 （4.6 mm×200 mm, 5 μm） column, by high performance liquid chromatography （HPLC） with methanol and 0.02 mol/L ammonium acetate as mobile phase, the flow rate was 1.0 mL/min. Gradient elution program was ： 6 %-40 % methanol, 2.8 %/min; 40 %-60 % methanol, 2.5 %/min; 60 % methanol, 3 min;6 % methanol,7 min. The wave length was 0-5.5 min, 430 nm; 5.51 min-8.0 min, 520 nm ; 8.01 min-10.0 min, 510 nm; 10.01 min-13min, 480 nm; 13.01 min-20 min, 600 nm. A method for extraction of synthetic colors in preserved fruit was developed. This method is accurate and anti-interfere with high recovery of synthetic colors.