PTEN、NF-κBp65和CyclinD1在胃腺癌中的表达及其与肿瘤临床病理间的相关性

Expressions of PTEN,NF-κBp65 and CyclinD1 in Gastric Adenocarcinoma and Their Correlations with Clinicopathologic of the Tumor

背景与目的:探讨PTEN、NF-κBp65和CyclinD1在胃腺癌组织中的表达及其与肿瘤临床病理特征之间的相关性。材料与方法:用免疫组织化学S-P法检测73例胃腺癌及20例正常胃粘膜组织中PTEN、NF-κBp65和CyclinD1的表达情况。结果:①PTEN在胃腺癌组织中蛋白表达阳性率为42.5%(31/73),明显低于它在正常胃粘膜组织中的阳性表达率90%(18/20)(P<0.01);NF-κBp65和CyclinD1在胃腺癌组织中蛋白表达阳性率分别为58.9%(43/73)和60.3%(44/73),明显高于它们在正常胃粘膜组织中的阳性表达率20%(4/20)和25%(5/20)(P<0.01);②PTEN与胃腺癌组织分化程度呈正相关(P<0.01),与胃腺癌浸润深度、淋巴结转移及临床分期呈负相关(P<0.01或P<0.05);NF-κBp65与胃腺癌组织分化程度呈负相关(P<0.05),与胃腺癌浸润深度、淋巴结转移及临床分期呈正相关(P<0.05或P<0.01);CyclinD1与胃腺癌的浸润深度相关(P<0.05),与组织分化程度、淋巴结转移及临床分期无相关关系;③胃腺癌中PTEN的阳性表达率与NF-κBp65和CyclinD1均呈负相关(P<0.05,P<0.01),NF-κBp65与CyclinD1的阳性表达率呈正相关(P<0.05)。结论:PTEN、NF-κBp65和CyclinD1基因在胃腺癌的发生、发展中起着不同的作用;联合检测PTEN、NF-κBp65和CyclinD1,可能有助于对胃腺癌恶性程度的判定及侵袭转移能力的评估,进而为胃腺癌的预后分析和进一步治疗提供依据。

BACKGROUND ＆ AIM： To investigate the protein expressions of PTEN, NF-κBp65 and CyclinD1 in gastric adenocarcinoma and the relationship between their expressions and clinicolpathological features. MATERIALS AND METHOd： Protein expressions of PTEN, NF-κBp6 and CyclinD1 in paraffin embedded tissues from 73 cases of gastric adenocarcinoma and 20 normal gastric mucosa tissues were analyzed by immunohistochemical method. RESULTS：①The positive rate of PTEN protein expression was 42.5% （31/ 73） in gastric adenocarcinoma, lower than that in normal gastric mucosa tissues 90% （18/20）（P〈 0.01）; The positive rates of NF-κBp65 and CyclinD1 protein expression were 58.9% （43/73）and 60.3% （44/73）,respectively in gastric adenocarcinoma, higher than those in normal gastric mucosa tissues 20% （4/20）and 25% （5/20）, respectively（both P〈0.01）; ②The expression of PTEN in well-differentiated adenocarcinoma was higher than that in poorly-differentiated adenocarcinoma （P 〈 0.01） . Also, the loss or decreased expression of PTEN significantly correlated with infiltrative depth, lymph node metastasis and clinicolpathological stage （P〈 0.01 or P 〈 0.05） . Contrary to PTEN, the expression level of NF-κBp65 in poorly-differentiated adenocarcinoma was higher than that in well-differentiated ones（P〈0.05）, and the increased expression of NF-κBp65 significantly correlated with infiltrative depth, lymph node metastasis and clinicolpathological stage（P〈0.05 or P〈0.01） .The expression level of CyclinD1 only correlated with infiltrative depth of gastric adenocarcinoma（P〈0.05）; ③Significantrelationships were found between FFEN and NF-κBp65 or CyclinDl（P 〈0.05, P 〈0.01）, while a positive correlation could also be found between NF-κBp65 and CyclinD1（P 〈0.05） . CONCLUSION： This expreiment suggested that FTEN, NF-κBp65 and CyclinD1 may play certain roles in the oncogenesis and progression of gastric adenocarcinoma, but to different extents. Combined evaluation of FTEN, NF-κBp65 and CyclinD1 may be helpful to assess the malignant degree, treatment and prognosis of gastric adenocarcinoma.