脑型肌酸激酶同工酶测定在非心源性疾病的应用

The Application of Detecting Brain Type Isoenzyme of Creatine Kinase in Non-Cardiovascular Diseases

目的探讨脑型肌酸激酶同工酶(CK-BB)在肿瘤、自身免疫性疾病的筛查以及器官损伤定位中的意义。方法免疫抑制法检测血清标本心肌型肌酸激酶同工酶(CK-MB)、肌酸激酶(CK)活性,计算二者比值。随机选取CK-MB/CK>50%的非心源性疾病的血清标本90份及30份对照血清,用CK同工酶琼脂糖凝胶电泳进行分离、鉴定。结果血清CK-MB假性增高的标本多见于肿瘤、自身免疫病,经电泳分离及可见光扫描后,肿瘤和自身免疫性疾病组的CK-BB含量明显高于对照组,差别具有统计学意义(P<0.01),血清CK-BB增高情况与其在组织器官中的分布相符。结论血清CK-BB的检测在肿瘤、自身免疫性疾病的辅助诊断以及器官损伤定位中具有重要意义。

Objective To discuss the value of detecting CK-BB in evaluating the injured organs of non-cardiovascular diseases. Methods Activates of CK-MB and total CK were tested using immunoinhibiton method and the ratios of CK-MB and CK were calculated. Clinical analysis of 90 non-cardiovascular patients with CK-MB/CK〉50% and 30 normal serve as control. Results Significantly increased serum levels of CK-BB were found in 90 samples with abnormal elevating CK-MB compared to controls. The differences in two groups were significant（P〈0.01）. The increased serum level of CK-BB is conforming to its content in different tissue that had profund significant in cases of non-cardiovascular CK-MB/CK〉 50 %. Conclusion The detection of CK-BB in serum is of great importance in evaluating injured organs of non-cardiovascular diseases.