水溶性氮酮对体外培养兔眼晶体上皮细胞活性及超微结构的影响

Effect of Water Soluble Azone on the Viability and Ultrastructure of Rabbit Lens Epithelial Cells

目的研究水溶性氮酮对体外培养兔眼晶体上皮细胞活性及超微结构的影响,探索水溶性氮酮的眼部安全应用浓度。方法体外培养兔眼晶体上皮细胞,采用细胞记数法、MTT法、透射电子显微镜研究0.001,0.005,0.010,0.050,0.100,0.500,1.000 g.L-1水溶性氮酮对细胞活性和超微结构的影响。结果浓度<0.100 g.L-1的水溶性氮酮作用24 h对晶体上皮细胞的活性无影响;浓度≥0.500 g.L-1的水溶性氮酮作用24 h可引起晶体上皮细胞活性下降(P<0.01)。浓度<0.100 g.L-1水溶性氮酮作用10 min对细胞器等结构无损害。浓度≥0.500 g.L-1的水溶性氮酮可导致细胞死亡。结论水溶性氮酮在浓度0.100 g.L-1对不会导致细胞活性的降低及细胞器损害,有较大的临床应用价值。

Objective To study the effect of water soluble azone on the viability and ultrastructure of rabbit lens epithelial cells cultured in vitro in order to probe into the safety application concentration of the drug for the eye in the clinical practice. Methods Rabbit lens epithelial cells were cultured in vitro. The cells were exposed to aqueous solution of azone in concentrations of 0.001, 0.005, 0. 010, 0.050, 0. 100, 0.500 and 1. 000 g · L ^-1 , respectively. Viability and ultrastructure of the cells were then assayed with cell counting, MTT method and scanning as well as transmission electron microscopy, respectively. Results Viability of the rabbit lens epithelial cells was not affected after they had been exposed to water soluble azone in concentrations lower than 0.10 g· L^-1 for 24 h. A decline in the viability of cells was noticed after a 24 h exposure of cells to water soluble azone in concentrations higher than 0. 500 g ·L^-1 （P 〈0.01 ）. Organelles of the lens epithelial cells were not injured after a 10 minute exposure of the cells to water soluble azone in concentrations lower than 0. 100 g · L ^-1, as evidenced under the transmission electron microscope . Water soluble azone in concentrations ≥0. 500 g · L^-1, however, was shown to cause extensive cell death. Conclusion Water soluble azone in concentrations no higher than 0. 100 g· L^-1 are unlikely to cause decline in viability of and injury of organelles in lens epithelial cells. Aqueous solution of azone in these concentrations, therefore, is of considerable application value to the clinical practice.