雌激素受体XbaⅠ和PvuⅡ基因多态性与慢性牙周炎的相关研究

Correlationship between the estrogen receptor gene XbaI and PvuⅡ polymorphism and the chronic periodontitis

目的:了解慢性牙周炎易感性与雌激素受体基因多态性的关系。方法:收集55例中、重度慢性牙周炎患者和60例牙周健康的对照组的颊黏膜拭子,采用Chelex-100法提取DNA,应用PCR-RFLP检测雌激素受体基因的基因型分布。结果:中、重度慢性牙周炎组与对照组在XbaⅠ和PvuⅡ基因型分布上均无统计学意义;多元因素分析结果年龄的OR值为1.128(95%CI:1.079,1.179),即年龄每增加1岁,患中、重度慢性牙周炎的可能性变为1.128倍。当其他条件一定,Xx基因型的人患中、重度慢性牙周炎的可能性是XX基因型的0.187倍,xx基因型的人患重度慢性牙周炎的可能性是XX基因型的0.421倍。结论:中、重度慢性牙周炎易感性与雌激素受体XbaⅠ基因型分布有关,XX基因型最易患病,其次为xx,Xx。

Objective：To study the relationship between the susceptibility of C P and the estrogen receptor gene polymorphism, nethods：DNA was isolated from 55 patients＇buccal swabs with aggressive periodontitis and extracted by Chelex-100. ER gene polymorphisms were assessed by PCR amplification and digestion with Pvu Ⅱ 和 Xba Ⅰ restriction enzyme respectively. Genotype distribution was compared between control group and the CP group. Results：The distributions of Xba Ⅰ and Pvu Ⅱ genotype in CP and control group showed no statistical differences（ P 〉0.05 ）. According to the logistic regression, the OR of age was 1. 128 which showed that when age increase 1 year, the possibility of contracting CP was higher. The OR of genotype was 0. 187 and 0.421, which showed that people with XX genotype were also more susceptible than those with Xx and xx genotype, when the other conditions were invariable. Conclusion：Specific relationship is found between the susceptibility of severe CP and the ER gene-XbaⅠ pelymorphism, People with XX genotype are most susceptive to CP.