产鸟苷的枯草杆菌缺失GMP还原酶活性突变株的选育

Breeding of Bacillus subtilis G-205 with Lack-GMP Reductase for Production of Guanosine

以枯草杆菌SM—12—2为出发菌株，经物理化学诱变剂连续处理，获得一株8一氮杂鸟嘌呤（8—AG），缺失鸟苷酸（GMP）还原酶活性的突变株G—205。该突变株肌苷（IMP）脱氢酶活性比亲株高，在培养基中积累5．17mg／ml鸟苷，9．84mg／ml肌苷。

A inosine producing strain of Bacillus subtilis SM-12-2 were mutated by UV-ray,EMS and NTG repeatedly, then a strain of Bacillus subtilis G-205 with 8-azaguanine resis-tant and lack-guanylic acid (GMP ) reductase activity were obtained.The inosiric acid(IMP) debydrogenase activity in the strain of B. subtilis G-205 was highter than that ofparent strain. The amount of guanosine and inosine which were accumunted by this mutantin the fermention culture was 5. 17mg/ml and9.84mg/nl respectively.