人大肠癌细胞中内质网分子伴侣Grp94与c-myc癌基因在表达上的相关性

Closely relationship between expression of endoplasmic reticulum molecular chaperone Grp94 and c myc oncogene in human colorectal carcinoma cell lines

探讨人大肠癌细胞中表达缺失性内质网分子伴侣Ｇｒｐ９４产物（Ｇｒｐ９４β）的生物学意义。方法应用地高辛标记ｃ－ｍｙｃｃＤＮＡ探针的斑点杂交，和以Ｇｒｐ９４基因近３′端一对寡核苷酸为引物的ＲＴ－ＰＣＲ技术，比较了人大肠癌细胞系ＣＣＬ２２９、ＣＸ－１以及经维甲酸诱导的ＣＣＬ２２９细胞中Ｇｒｐ９４和ｃ－ｍｙｃ癌基因在表达上的关系。结果Ｇｒｐ９４和ｃ－ｍｙｃ癌基因在ＣＣＬ２２９细胞中的表达水平明显高于ＣＸ－１细胞，且随维甲酸诱导ＣＣＬ２２９细胞时间的延长，二者呈一致性地下降，并于诱导后第６天降至最低水平；而原来于ＣＣＬ２２９细胞中检测到的Ｇｒｐ９４基因的正常表达产物（Ｇｒｐ９４α），随维甲酸诱导时间的延长逐渐增加。结论提示Ｇｒｐ９４和ｃ－ｍｙｃ癌基因产物之间可能存在一定联系。

Objective To study the biological significance of Grp94 deleted product(Grp94β) expressed in human colorectal carcinoma cells. Methods The relationship between molecular chaperone Grp94 and c myc oncogene expression in the human colorectal carcinoma cell line CCL229, CX 1 and retinoic acid (RA) induced CCL229 by both of digoxin labelled c myc cDNA probe spot bybridization and RT PCR with endoplasmic reticulum molecular chaperone Grp94 proximal 3′ end oligonucletide primers was investigated. Results In CCL229 cells, the expression of Grp94β and c myc oncogene are significantly higher than those in CX 1 cells. Along with the RA inducing, both decreased with one accord, and to the lowest level after six days induction. Conclusion The Grp94 normal expression (Grp94α), which was undetectable in CCL229 cells increased after RA induction. The results showed that the expression of Grp94β may be closely related to that of c myc oncogene. It is suggested that the Grp94 be related to some biological characteristics of cancer, for example, the invasiveness.