星形胶质细胞在cuprizone介导的急性脱髓动物模型中的变化及其意义

Changes and significance of astrocyte in cuprizone-induced acutely demyelinated animal model

目的:利用cuprizone制备髓鞘可再生的急性脱髓鞘动物模型,探讨脱髓鞘疾病髓鞘再生机制。方法:在饲料中掺入cuprizone饲育小鼠,通过调控饲育时间,造成神经脱髓鞘及髓鞘再生,并利用免疫荧光染色和原位杂交方法,检测不同时间点髓鞘的脱失和再生情况以及星形胶质细胞的反应。结果:cuprizone饲育6周后,动物小脑和胼胝体白质内髓鞘脱失严重,星形胶质细胞增殖活跃;在恢复正常饲料后,髓鞘基本恢复正常结构,星形胶质细胞仍处于活化增殖状态。结论:利用cuprizone可制备髓鞘可再生的急性脱髓鞘动物模型,星形胶质细胞的活化增殖对髓鞘再生具有重要作用。

Objective： To explore mechanism of demyelination and remyelination in cuprizone-induced acutely demyelinated animal model. Methods： Acutely demyelinated animal model was established by feeding C57BL/6 mice with cuprizone in the diet. Using immunohistochemistry and In situ hybridization, we demonstrated status of demyelination and remyelination and reaction of astrocyte at different time-points, Results： Severe demyelination as well as proliferation of astrocyte occurred in corpus callosum and cerebellum following 6 weeks exposure to cuprizone, while myelin became normal by remyelination after the mice were returned to 4 weeks of normal diet, and proliferation of astrocyte still retained. Conclusions： Cuprizone-induced acutely demyelinated animal model can be established successfully, and activated astrocyte appears to contribute to remyelination.