人血清中青霉素抗体的酶联免疫吸附测定

Detection of penicillin antibodies in human serum by enzyme-linked immunosorbent assay

目的:建立检测青霉素抗体的ELISA法,并探讨其临床意义。方法:以氨苄西林用SPDP法与兔血清白蛋白交联,用此交联物为包被抗原,HRP-抗人IgG为酶标抗体,建立检测青霉素抗体的ELISA法,并对部分临床标本进行青霉素抗体检测。结果:建立的方法其批内、批间变异系数分别为6.8%、8.4%。氨苄西林-兔白蛋白交联物免疫家兔获得兔抗氨苄西林的抗体,以此抗体作阻断试验,抑制HRP-抗人IgG的显色。临床标本检测结果表明,不同疾病患者青霉素抗体的阳性率不同。结论:ELISA可用于临床对青霉素抗体的检测,具有较好的特异性、敏感性和重复性。

Objective：To establish an enzyme-linked immunosorbent assay（ELISA）for detection of the antibody of penicillin in human serum.Methods：The ELISA to detect the penicillin antibody was established by adding sera into the polystyrene micro-titer plates coated with penicillin-conjugated rabbit serum albumin and HRP-conjugated goat anti-human IgG.The Penicillin antibody in some clinical samples was detected with this method.Results：The within and between assay CV were 6.8% and 8.4% respectively.The rabbit anti-penicillin antibody,which was obtained from penicillin-conjugated rabbit serum albumin,could control the HRP-anti human IgG reaction.The positive rate of penicillin antibody in patients with different diseases was significantly different.Conclusions：ELISA is more sensitive and specific in clinically detecting and antibody of penicillin.