摘要
目的:研究建立测定血浆中复方氯唑沙宗片两组分浓度的HPLC-UV法。方法:反相高效液相色谱法测定血浆中药物的浓度。Zorbax XDB-C18色谱柱,血浆样品经乙醚提取,空气吹干,加流动相溶解进样。氯唑沙宗的流动相为甲醇∶水=(60∶40),在波长282nm处检测;对乙酰氨基酚的流动相为甲醇∶水=(20∶80),在波长245nm处检测,以外标法计算。结果:氯唑沙宗在0.208~33.28μg/ml、对乙酰氨基酚在0.238~14.28μg/ml浓度范围内,样品的峰面积与对应浓度呈良好线性关系,r氯=0.9998,r对=0.9998。最低检测限均为0.1μg/ml,氯唑沙宗高、中、低(20.8μg/ml,4.16μg/ml,0.416μg/ml)3种浓度的方法回收率分别为(103.36±6.0)%,(99.51±1.2)%,(99.08±1.88)%;对乙酰氨基酚高、中、低(9.52μg/ml,2.38μg/ml,0.476μg/ml)3种浓度的方法回收率依次为(101.5±3.15)%,(97.6±1.93)%,(99±3.15)%,日内RSD分别为(1.2%~2.3%)、(1.4%~6.7%),日间RSD分别为(2.5%~5.1%)、(5%~7.8%)。结论:此法操作简便、结果可靠。
Objective:To establish a HPLC method for the determination of two component concentrations of complex chlorzoxazone tablets in human serum.Methods:The serum samples were extracted with ether,dried with gas,dissolved in mobile phase and determined with HPLC. The Zorbax XDB-C18 column was used. The mobile phase consisted of methanol:water (60:40)or methanol:water (20:80) respectively. It was detected at 282 nm or 245nm respectively.Results:A hnearity was obtained from 0.208 to 33.28 μg/ml of chlorzoxazone (r=0.9998) and from 0.238 to 14.28 μg/ml of acetaminophen (r = 0.9998) respectively. The limit of detection was 0.1 μg/ ml. Their average recoveries were 95.3% and 99.4% respectively. The precision (RSD) was 6.0% and 2.4% respectively. Conclusion: This study provided a simple and reliable assay which can be used to determine two component concentrations of complex chlorzoxazone tablets in human serum.
出处
《现代医药卫生》
2007年第19期2856-2857,共2页
Journal of Modern Medicine & Health