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ANKRD17蛋白抗体的制备、纯化及检测 被引量：2

Preparation,Purification the Antibody and Immunofluorescence Analyse of ANKRD17
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摘要 目的:制备ANKRD17(P260)蛋白的兔多克隆抗体,以与抗原相结合的方法进行抗体的纯化,并利用纯化的抗体对该蛋白进行细胞内免疫荧光检测。方法:构建表达GST-ANKRD17C端融合蛋白的质粒,在大肠杆菌中诱导表达;制备GST-ANKRD17C端抗原融合蛋白后免疫家兔,对获得的兔多克隆抗血清进行亲和纯化;纯化后的抗体经过Westernblot鉴定,用于细胞免疫荧光染色检测。结果:获得较高效价的血清抗体,并对血清抗体进行了纯化;利用纯化的抗体对ANKRD17蛋白进行了细胞内免疫荧光检测,发现改蛋白定位于细胞质中。结论:制备得到的纯化抗体为研究ANKRD17蛋白的功能打下了必要的基础。 Objective： To obtain special anti-ANKRD17 polyclonal antibodies and analyse the localization of ANKRD17 in cells by immunofluorescence. Methods： GST-ANKRD17 C terminal plasmid was construted and expressed in E.coli BL21. The rabbits were immunizedand with the purified antigens, and the serum were collected and purified by affinity purification. The purified special antibodies were identified by Western blot and be used to analyse the localization of ANKRD17 in cells by immunofluorescence. Results： The high titer antiserum purified special antibodies were obtained. The purlfed special antibodies were successfully used to immunofluorescence analyse. Conclusion： The purifed special antibodies will benefit the study of the ANKRD17 portein＇s function.
作者 舒伟 李发慧 邓敏 潘全 马清钧 叶昕
机构地区 军事医学科学院生物工程研究所 中国科学院微生物研究所
出处 《生物技术通讯》 CAS 2007年第5期771-773,共3页 Letters in Biotechnology
关键词 ANKRD17蛋白 亲和纯化 免疫荧光 ANKRD17 portein affinity purification immunofluorescence
分类号 Q25 [生物学—细胞生物学] R392.1 [医药卫生—免疫学]
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生物技术通讯
2007年 第5期

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