航天诱变凤仙花总RNA的提取及RT-PCR初探

The Total RNA Extraction and RT-PCR of Mutants of Impatiens balsamina Induced by Space Flight

目的:为研究凤仙花的突变性状,克隆相关突变基因,探讨了凤仙花总RNA的提取方法,并利用RT-PCR克隆花色调控基因。方法:对加拿大BBI和日本TaKaRa公司提供的RNA提取试剂盒进行实验比较并适当改良,提取高质量的凤仙花总RNA。结果:提取到较高质量的凤仙花总RNA,克隆了其花色调控基因。结论:用改进的方法提取的总RNA质量较好,能用于基因克隆等相关实验。

Objective： In order to investigate the characteristics and clone the related genes in mutants of Impatiens balsamina, the total RNA extraction methods were studied. Methods： Based on the comparison of the techniques provided by Canadian BBI Corporation and Japanese TaKaRa Corporation, a modified method for RNA extraction was established, high quality of total RNA was extracted. Results： The extracted RNA could be used for RT-PCR analysis and cloning some gene fragments relative to flower color. Conclusion： The modified method is fit for the extraction of total RNA from I. balsamina, and RNA could be used for gene cloning.